A9941
Ácido 2,2′-acino-bis(3-etilbenzotiazolina-6-sulfónico) diammonium salt
chromogenic, tablet
Sinónimos:
AzBTS-(NH4)2, Diamonio del 2,2′-acino-bis(3-etilbenzotiazolina-6-sulfonato)
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About This Item
Fórmula empírica (notación de Hill):
C18H24N6O6S4
Número CAS:
Peso molecular:
548.68
NACRES:
NA.21
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
250-396-6
MDL number:
Beilstein/REAXYS Number:
7329461
Nombre del producto
Ácido 2,2′-acino-bis(3-etilbenzotiazolina-6-sulfónico) diammonium salt, tablet, 10 mg substrate per tablet
InChI key
OHDRQQURAXLVGJ-AXMZSLBLSA-N
InChI
1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;
SMILES string
[NH4+].[NH4+].CCN1C(\Sc2cc(ccc12)S([O-])(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S([O-])(=O)=O
form
tablet
solubility
deionized water: 100 mL
storage temp.
room temp
Quality Level
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Application
El ácido 2,2′-acino-bis(3-etilbenzotiazolina-6-sulfónico es un sustrato de la peroxidasa adecuado para procedimientos ELISA. Este sustrato rinde un producto final soluble que es de color verde y puede leerse espectrofotométricamente a 405 nm. La reacción puede pararse con dodecilsulfatosódico (SDS) al 1 %. Recomendado para procedimientos ELISA (micropocillo), no recomendado para aplicaciones con membrana.
- 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, ABTS, has been used as a substrate in ELISA (enzyme-linked immunosorbent assay).†
- ABTS is also used to measure glucose concentrations in different solutions including blood serum.
- The food industry uses ABTS to measure the antioxidant capacities within different foods.
- Since it is less readily oxidized than TMB and OPD substrates, ABTS is less sensitive for ELISA applications. Thus ABTS has some advantageous over TMB and OPD in cases of large background results
- It is recommended for ELISA (microwell) procedures, not recommended for membrane applications.
General description
ABTS mainly used in ELISA (enzyme-linked immunosorbent assay) procedures.
ABTS, 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), is a chromogenic substrate used:
ABTS, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt is light sensitive, and water soluble chemical compound
ABTS, 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), is a chromogenic substrate used:
- For a reaction with laccase or bilirubin oxidase
- For a reaction with peroxidase enzymes: (like HRP for example). ABTS in combination with hydrogen peroxide
ABTS, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt is light sensitive, and water soluble chemical compound
Preparation Note
Dissolve 1 tablet in 100 ml of 0.05 M phosphate-citrate buffer, pH 5.0 (Tablets available as Sigma Product No. P4809). Add 25 μl of fresh 30% hydrogen peroxide
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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B Porstmann et al.
Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie, 19(7), 435-439 (1981-07-01)
o-Phenylenediamine, 2,2'-azino-di(3-ethylbenzthiazoline sulphonic acid-6) (ABTS), o-dianisidine and 4-aminoantipyrine were compared as chromogens for the determination of horseradish peroxidase. Highest sensitivity in the determination of horseradish peroxidase-IgG conjugates in dissolved form was obtained with o-phenylenediamine. When these conjugates were used in
Humoral and Cell-Mediated Immune Response in Colostrum from Women Diagnosed Positive for SARS-CoV-2.
Vignesh Narayanaswamy et al.
Breastfeeding medicine : the official journal of the Academy of Breastfeeding Medicine, 16(12), 987-994 (2021-08-13)
Objective: To evaluate the immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in colostrum from women who tested positive for the virus. Methods: Between March and September 2020 we obtained bilateral colostrum samples collected on spot cards within
Collaborative study on a guinea pig serological method for the assay of acellular pertussis vaccines
Winsnes R, et al.
Pharmeuropa Bio & Scientific Notes, 19-32 (2009)
Development of a feline proinsulin immunoradiometric assay and a feline proinsulin enzyme-linked immunosorbent assay (ELISA): A novel application to examine beta cell function in cats
Kley S, et al.
Domestic Animal Endocrinology, 311-318 (2008)
Da Di et al.
Pathogens (Basel, Switzerland), 10(8) (2021-08-29)
The SARS-CoV-2 nucleocapsid protein (N) binds a single-stranded viral RNA genome to form a helical ribonucleoprotein complex that is packaged into virion particles. N is relatively conserved among coronaviruses and consists of the N-terminal domain (NTD) and C-terminal domain (CTD)
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