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Merck

F7425

ANTI-FLAG® antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Sinónimos:

Anti-ddddk, Anti-dykddddk

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.32
MDL number:
MFCD02262912

Nombre del producto

ANTI-FLAG® antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

all

concentration

~0.8 mg/mL

technique(s)

dot blot: 1-2.5 μg/mL
immunoprecipitation (IP): 4-8 μg using amino terminal FLAG-BAP fusion protein from E. coli crude lysate
indirect immunofluorescence: 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK
western blot (chemiluminescent): 1-2.5 μg/mL using an E. coli periplasmic extract expressing an N-terminal FLAG fusion protein

isotype

IgG

immunogen sequence

DYKDDDDK

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

Categorías relacionadas

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Application

El anticuerpo reconoce el epítopo FLAG situado en las proteínas de fusión etiquetadas con FLAG en los extremos N-terminal o C-terminal mediante la aplicación de ensayos de inmunotransferencia por puntos (dot blot), inmunotransferencia, inmunoprecipitación e inmunocitoquímica.
Conozca más detalles del producto en nuestro portal de la aplicación FLAG®.

Biochem/physiol Actions

El epítopo FLAG desempeña un papel crucial en la purificación mediante inmunoafinidad de las proteínas de fusión.

General description

El anticuerpo policlonal de afinidad anti-FLAG de conejo ANTI-FLAG reconoce el epítopo FLAG situado en las proteínas de fusión FLAG. Este anticuerpo reacciona con los extremos N-terminal, Met-N-terminal, C-terminal de las proteínas de fusión FLAG.
El epítopo FLAG es una proteína de ocho aminoácidos con un sitio de escisión por enterocinasa.

Immunogen

FLAG; secuencia peptídica DYKDDDDK

Other Notes

Ganador del premio CiteAb 2024 como proveedor de éxito en la investigación del Parkinson

Physical form

Disolución en disolución salina tamponada con fosfato 0,01 M, pH 7,4, que contiene seroalbúmina bovina al 1 % y acida sódica 15 mM.

Preparation Note

Purificado mediante cromatografía de afinidad en una columna portadora del péptido inmunizante.

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Clase de almacenamiento

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000485141
0000485141
0000422515
0000422515
0000395474

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  1. Is Product F7425, ANTI-FLAG® from rabbit, a monoclonal or polyclonal?

    F7425 is a polyclonal antibody from rabbit.

  2. What is the concentration of Product F7425, ANTI-FLAG® from rabbit?

    The concentration is 0.9 mg protein per ml.

  3. How much Product F7425, ANTI-FLAG® from rabbit, should be used in western blotting and ELISA applications?

    For a Western blot from a PAGE run (preferably) under denaturing conditions, a 1:400 dilution of this antibody should be used.  A 1-10 μg/ml concentration should be used when using thie antibody in an ELISA application.

  4. How to get rid of background when using Product F7465, ANTI-FLAG® from rabbit,?

    To reduce non-specific background on tissue staining, a small amount of Triton X-100 (Product No. X100 or T8787), can be added to the wash buffers and antibody incubation buffers. Try a Triton X-100 concentration of 0.1% to start. Tween 20 (Product No. P5927) can also be used.

  5. Is there any references for the use of Product F7465, ANTI-FLAG® from rabbit, in immunohistochemistry or western blotting?

    K. Schafer and T. Braun, Monoclonal Anti-FLAG Antibodies React with a New Isoform of Rat Mg++ Dependent Protein Phosphatase. Biochemical and Biophysical Research Communications, 207, 708.

  6. What are the positive controls that can be used with Product F7425, ANTI-FLAG® from rabbit?

    The positive controls that can be used are as follows:Amino-terminal FLAG-BAP fusion protein, Product No. P7582Carboxy-terminal FLAG-BAP fusion protein, Product No. P7457Amino-terminal Met-FLAG-BAP fusion protein, Product No. P5975.

  7. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  8. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  9. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  10. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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