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Merck

M5655

Azul de tiazolil Bromuro de tetrazolio

powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)

Sinónimos:

Bromuro de 3-(4,5-dimetil-2-tiazolil)-2,5-difenil-2H-tetrazolio, Bromuro de metiltiazolildifenil-tetrazolio, MTT

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About This Item

Fórmula empírica (notación de Hill):
C18H16BrN5S
Número CAS:
298-93-1
Peso molecular:
414.32
UNSPSC Code:
12352207
NACRES:
NA.75
PubChem Substance ID:
24897030
EC Number:
206-069-5
Beilstein/REAXYS Number:
4081397
MDL number:
MFCD00011964

Nombre del producto

Azul de tiazolil Bromuro de tetrazolio, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)

InChI key

AZKSAVLVSZKNRD-UHFFFAOYSA-M

InChI

1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1

SMILES string

[Br-].Cc1nc(sc1C)-[n+]2nc(nn2-c3ccccc3)-c4ccccc4

product line

BioReagent

assay

≥97.5% (HPLC)

form

powder

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable

mp

195 °C (dec.) (lit.)

solubility

H2O: 5 mg/mL

storage temp.

2-8°C

Quality Level

200

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Application

El azul de tiazolil Azul tetrazolio (MTT) puede utilizarse para medir la proliferación celular. El MTT produce una disolución amarillenta que vira a formazano de MTT azul violeta insoluble en agua por acción de las deshidrogenasas mitocondriales de las células vivas. Los cristales azules se solubilizan con isopropanol acidificado y la intensidad se mide colorimétricamente a 570 nm.
El azul de tiazolil Azul tetrazolio (MTT) puede utilizarse para medir la proliferación celular. El MTT produce una disolución amarillenta que vira a formazano de MTT azul violeta insoluble en agua por acción de las deshidrogenasas mitocondriales de las células vivas. Los cristales azules se solubilizan con isopropanol acidificado y la intensidad se mide colorimétricamente a 570 nm. El MTT se ha utilizado como un reactivo histoquímico o citoquímico y para la detección del NAD. Los sistemas enzimáticos ligados a ADP de los tejidos no pueden detectarse con MTT, debido a la unión del catión por la trampa de cianuro utilizada. El MTT se reduce rápidamente al formazano, que forma complejos con el níquel, el cobre y el cobalto; los quelatos de cobalto se han utilizado en los sistemas oxidativos.
El bromuro de tetrazolio de azul de tiazolilo se ha utilizado como indicador colorimétrico de la actividad metabólica en análisis de viabilidad celular (análisis MTT). Se ha utilizado también para determinar la proliferación celular.

Other Notes

Ganador del premio CiteAb 2024 como proveedor de éxito en la investigación del Parkinson

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
MKCS4540
MKCR1832
MKCN5605
MKCL1832
MKCG3023

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. What can be used to make a solution of Thiazolyl Blue Tetrazolium Bromide (MTT)?

    We test the solubility of MTT in water at a concentration of 5 mg/mL.  For the MTT viability assay, we dissolve Product No. M5655 at a concentration of 5 mg/ml in RPMI-1640 without phenol red. This medium is available as a powder (Product No.R8755) or liquid (Product No. R7509). MTT can be solubilized in any culture media or balanced salt solution that does not contain phenol red as a pH indicator.

  3. How can I store solutions of Thiazolyl Blue Tetrazolium Bromide (MTT)?

    Reconstituted MTT solution is stable for at least 6 months when stored frozen (-20 °C). Storage of reconstituted MTT solution at 2-8°C for more than 2 weeks may cause decomposition and yield erroneous results.

  4. What can be used to solubilize MTT formazan (the product of the Thiazolyl Blue Tetrazolium Bromide - MTT reaction)?

    Mitochondrial dehydrogenases of viable cells cleave the tetrazolium ring of the MTT, yielding purple MTT formazan crystals. These crystals are not soluble in aqueous solutions; they may be dissolved in acidified isopropanol (0.1 N HCl in anhydrous isopropanol). If this does not lyse the cells, Triton X-100 can be added to the acidified isopropanol. A typical working concentration is 10% Triton X-100 (i.e., 1 volume of 100% Triton X-100 + 9 volumes of acidified isopropanol).

  5. Why is a reference wavelength used for data analysis of Thiazolyl Blue Tetrazolium Bromide (MTT)?

    The reference wavelength (630-690 nm) is used to adjust for the optical variation in the wells of the plate.  It also corrects for dust and fingerprints that can be present.  The assay is read at 570 nm.  The reference wavelength needs to be far enough away from the assay wavelength so as not to affect those values.

  6. What type of multi-well plate should be used for the Thiazolyl Blue Tetrazolium Bromide (MTT) assay?

    It is recommended that flat bottom multi-well plates be used for the MTT assay. The use of tissue culture treated plates is only important if necessary for good cell growth.  If cells are grown in larger plates, the supernatant after cell lysis can be transferred to a 96 well plate for reading.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. Is the MTT assay quantitative?

    The MTT asay is a method to assess cell viability.  This assay is a semiquantitative assay. The assay is used to compare the viability changes in treated cells to untreated cells. The absorbance is indicative of the cell number. The higher the absorbance, the greater the number of viable cells present. Most researchers compare the absorbance of the two samples as a ratio (ABS treated cells/ABS untreated cells) to get a fold increase/decrease in cell number.   

  11. How can Thiazolyl Blue Tetrazolium Bromide be used for staining dehydrogenase in tissue sections?

    Products M2128 or M5655, Thiazolyl Blue Tetrazolium Bromide can be used for staining dehydrogenase in tissue sections. See the method below. Reagent PreparationMTT (2 mg/ml distilled water) 2.5 ml0.2 M Tris buffer, pH 7.4 2.5 ml0.5 M cobalt chloride 0.5 ml0.05 M magnesium chloride 1.0 mlDistilled water 2.5 mlThe pH of this solution is adjusted to 7.0 if necessary. Aliquot and store at -20°C until required. Sample PreparationCut cytostat sections at 5 to 7 microns. Do not fix. Method:1. Incubate sections in appropriate incubation solution at 37°C for 30-60 minutes.2. Transfer sections to 15% formol saline, 15 minutes.3. Wash in distilled water4. Counterstain in 2% methyl green if required.5. Wash in distilled water.6. Mount in glycerin Jelly. ResultsEnzyme: Black formazan deposits with MTT Source:  Bancroft and Gamble, Theory and Practice of Histological Techniques, Fifth Edition. Churchill Livingstone, London, 2002, page 610.

  12. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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