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Merck

P8340

Cóctel de inhibidores de proteasas

DMSO solution, for the inhibition of serine, cysteine, acid proteases and aminopeptidases, for use with mammalian cell and tissue extracts, DMSO solution

Sinónimos:

Mezcla de inhibidor de proteasas

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.77
MDL number:
form:
DMSO solution
solubility:
water: soluble
storage temp.:
−20°C

Nombre del producto

Cóctel de inhibidores de proteasas, for use with mammalian cell and tissue extracts, DMSO solution

biological source

bovine lung

form

DMSO solution

solubility

water: soluble

storage temp.

−20°C

Quality Level

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Application

Ideal para utilizar como componente del tampón durante el tratamiento con ultrasonidos, tampón de lisis y tampón para RIPA

Proporciona protección contra la actividad enzimática endógena en extractos celulares y de tejidos

Biochem/physiol Actions

Esta mezcla contiene componentes individuales, como la AEBSF a 104 mM, aprotinina a 80 μM, bestatina a 4 mM, E-64 a 1,4 mM, leupeptina a 2 mM y pepstatina A a 1,5 mM. Cada componente tiene propiedades inhibidoras específicas. La AEBSF y la aprotinina actúan para inhibir las proteasas de serina, como la tripsina, la quimiotripsina y la plasmina, entre otras. La bestatina inhibe las aminopeptidasas. La E-64 actúa contra las proteasas de cisteína. La leupeptina actúa contra las proteasas de serina y de cisteína. La pepstatina A inhibe las proteasas ácidas.
Inhibe las proteasas de serina y cisteína, las proteasas ácidas y las aminopeptidasas.

Disclaimer

Este cóctel debe conservarse a -20 °C.

Features and Benefits

Inhibición de amplio espectro: inhibe múltiples clases de proteasas y aminopeptidasas

Conservación estable: se suministra como disolución en DMSO, lo que permite su conservación a largo plazo a -20°C

Rendimiento uniforme: disolución lista para usar

General description

Cóctel de inhibidores de proteasas para extractos de células y tejidos de mamíferos, en disolución DMSO. Contiene seis componentes individuales para inhibir las proteasas de serina, cisteína, las proteasas ácidas y las aminopeptidasas.

Other Notes

AEBSF
Aprotinina
Bestatina
E-64
Leupeptina
Pepstatina A

Preparation Note

Se recomienda 1 ml de esta disolución combinada para la inhibición de las enzimas endógenas que se encuentran en 100 ml de lisado procedente de 20 g de hígado bovino o 10 ml de lisado de células CHO. Este producto se suministra como una disolución en DMSO.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 2

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup


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Certificados de análisis (COA)

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  1. Is there anything in Product P8340, Protease Inhibitor Cocktail, that will cause downstream problems with RT-PCR?

    While the product should not cause any downstream problems with RT-PCR, we would advise to clean up the reaction with either DMN10 (GenElute Direct mRNA Miniprep Kit) or T9424 (TRI Reagent®) for  purification.

  2. Can Product P8340, Protease Inhibitor Cocktail, be used with RIPA buffer and how much should be used?

    Yes, this product can be used with RIPA buffer at 100 μl/ml concentration.

  3. How much Product P8340, Protease Inhibitor Cocktail, should I use?

    One mL of the cocktail solution is recommended for the inhibition of endogenous enzymes found in 100 mL of lysate from 20 g (wet weight) of bovine liver or 10 mL of cell lysate obtained from CHO cells at a cell density of 100 million (108) cells per mL. CHO cells were grown on DMEM with 10% FCS (heat inactivated).  Note: Not all lysates contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.

  4. What is the concentration of inhibitors in Product P8340, Protease Inhibitor Cocktail?

    The concentration of inhibitors in this product is the following:AEBSF, 104 mMAprotinin, 0.085 mMBestatin, 4 mME-64, 1.4 mMLeupeptin, 2 mMPepstain A, 1.5 mM.

  5. Does Product P8340, Protease Inhibitor Cocktail, contain any chelators or EDTA?

    This product does not contain any metal chelators.

  6. Can Product P8340, Protease Inhibitor Cocktail, be used with Ezview Protein A and Anti FLAG M2 affinity gels.

    Yes, this product can be used with both Ezview Protein A and Anti FLAG M2 affinity gels.

  7. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  8. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  9. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  10. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Signaling through extracellular signal-regulated kinase is required for spermatogonial proliferative response to stem cell factor.
Dolci S et al
The Journal of Biological Chemistry, 276(43), 40225-40233 (2001)
Interaction of Huntington disease protein with transcriptional activator Sp1.
Li SH et al
Molecular and Cellular Biology, 22(5), 1277-1287 (2002)
Megan D Schertzer et al.
Molecular cell, 75(3), 523-537 (2019-07-02)
Long noncoding RNAs (lncRNAs) cause Polycomb repressive complexes (PRCs) to spread over broad regions of the mammalian genome. We report that in mouse trophoblast stem cells, the Airn and Kcnq1ot1 lncRNAs induce PRC-dependent chromatin modifications over multi-megabase domains. Throughout the
A transgenic rat that develops Alzheimer's disease-like amyloid pathology, deficits in synaptic plasticity and cognitive impairment.
Liu L et al
Neurobiology of Disease, 31(1), 46-57 (2008)
Acceleration and persistence of neurofibrillary pathology in a mouse model of tauopathy following anesthesia.
Planel E et al
Faseb Journal, 23(8), 2595-2604 (2009)

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