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About This Item
Nombre del producto
Cóctel de inhibidores de proteasas, for use with mammalian cell and tissue extracts, DMSO solution
biological source
bovine lung
form
DMSO solution
solubility
water: soluble
storage temp.
−20°C
Quality Level
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Categorías relacionadas
Application
Proporciona protección contra la actividad enzimática endógena en extractos celulares y de tejidos
Biochem/physiol Actions
Disclaimer
Features and Benefits
Conservación estable: se suministra como disolución en DMSO, lo que permite su conservación a largo plazo a -20°C
Rendimiento uniforme: disolución lista para usar
General description
Other Notes
Aprotinina
Bestatina
E-64
Leupeptina
Pepstatina A
Preparation Note
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Clase de almacenamiento
10 - Combustible liquids
wgk
WGK 2
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
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Is there anything in Product P8340, Protease Inhibitor Cocktail, that will cause downstream problems with RT-PCR?
While the product should not cause any downstream problems with RT-PCR, we would advise to clean up the reaction with either DMN10 (GenElute™ Direct mRNA Miniprep Kit) or T9424 (TRI Reagent®) for purification.
Can Product P8340, Protease Inhibitor Cocktail, be used with RIPA buffer and how much should be used?
Yes, this product can be used with RIPA buffer at 100 μl/ml concentration.
How much Product P8340, Protease Inhibitor Cocktail, should I use?
One mL of the cocktail solution is recommended for the inhibition of endogenous enzymes found in 100 mL of lysate from 20 g (wet weight) of bovine liver or 10 mL of cell lysate obtained from CHO cells at a cell density of 100 million (108) cells per mL. CHO cells were grown on DMEM with 10% FCS (heat inactivated). Note: Not all lysates contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.
What is the concentration of inhibitors in Product P8340, Protease Inhibitor Cocktail?
The concentration of inhibitors in this product is the following:AEBSF, 104 mMAprotinin, 0.085 mMBestatin, 4 mME-64, 1.4 mMLeupeptin, 2 mMPepstain A, 1.5 mM.
Does Product P8340, Protease Inhibitor Cocktail, contain any chelators or EDTA?
This product does not contain any metal chelators.
Can Product P8340, Protease Inhibitor Cocktail, be used with Ezview Protein A and Anti FLAG M2 affinity gels.
Yes, this product can be used with both Ezview Protein A and Anti FLAG M2 affinity gels.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Contenido relacionado
EZviewTM Red Protein A and ANTI-FLAG® M2 Affinity Gels: Immunoprecipitation with Enhanced Visibility Affinity Beads - Technical Article - July 2001
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