S5761

Bicarbonato de sodio

Name: Bicarbonato de sodio
Brand: SIGMA

powder, BioReagent, Molecular Biology, suitable for cell culture, suitable for insect cell culture

Sinónimos:

Sodium hydrogen carbonate, soda bicarbonate

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About This Item

Fórmula lineal:

NaHCO₃

Número CAS:

144-55-8

Peso molecular:

84.01

UNSPSC Code:

12161702

NACRES:

NA.75

PubChem Substance ID:

24899673

EC Number:

205-633-8

Beilstein/REAXYS Number:

4153970

MDL number:

MFCD00003528

assay:

99.5-100.5%

grade:

Molecular Biology

form:

powder

solubility:

water: soluble 100 mg/mL

Nombre del producto

Bicarbonato de sodio, powder, BioReagent, Molecular Biology, suitable for cell culture, suitable for insect cell culture

InChI key

UIIMBOGNXHQVGW-UHFFFAOYSA-M

InChI

1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1

SMILES string

[Na+].OC([O-])=O

grade

Molecular Biology

product line

BioReagent

assay

99.5-100.5%

form

powder

quality

300

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable

pH

6.8-7.4

pKa (25 °C)

(1) 6.37, (2) 10.25 (carbonic acid)

solubility

water: soluble 100 mg/mL

density

2.16 g/mL at 25 °C (lit.)

Quality Level

300

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Application

Sodium bicarbonate has been used:

as a component of bovine embryo culture system during in vitro maturation, fertilization, and culture (IVMFC)
as a component of a 1:1 mixture of Dulbecco′s modified Eagle′s medium and Ham′s nutrient mixture F-12 during the culture of bovine microvascular endothelial cells
as a component of ITS (insulin transferrin and selenite) medium used during the isolation of dopamine neurons from human embryonic stem cells
as a component of medium used for cell culture of human embryonic kidney cells
as a component of Beltsville thawing solution used for the dilution of semen

General description

Sodium bicarbonate is broadly used as a physiological buffer in cell culture applications. The pKa for bicarbonate <-> carbonic acid reaction is 6.4 The pKa for bicarbonate <-> carbonate reaction is 10.3. Sodum bicarbonate has two pKas, 6.4 and 10.3. It is broadly used as an physiological buffer in in vitro applications.

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Ca2+ activation of hSlo K+ channel is suppressed by N-terminal GFP tag.

E Meyer et al.

The European journal of neuroscience, 11(3), 1105-1108 (1999-04-02)

The human slow poke (hSlo) K+ channel was tagged with GFP (green fluorescent protein) at the N-terminus of its alpha-subunit. The fusion protein was expressed transiently in HEK293 cells; it formed functional voltage-gated channels as shown by whole cell patch-clamp

Semen alterations in porcine rubulavirus-infected boars are related to viral excretion and have implications for artificial insemination.

M Solís et al.

Research in veterinary science, 83(3), 403-409 (2007-03-21)

Porcine rubulavirus (PoRV), also known as blue eye disease (BED) of swine, causes respiratory and reproductive problems in pigs at several developmental stages. To study the effect of PoRV infection on semen production, five boars were infected with 1 x

In vitro developmental competence of in vitro-matured bovine oocytes fertilized and cultured in completely defined media.

L Keskintepe et al.

Biology of reproduction, 55(2), 333-339 (1996-08-01)

The objective was to establish an in vitro system in which bovine oocytes can be matured, fertilized, and cultured up to the blastocyst stage without support of serum, BSA, or somatic cells. Media consisted of modified tissue culture medium 199

Tumor necrosis factor alpha receptors in microvascular endothelial cells from bovine corpus luteum.

K Okuda et al.

Biology of reproduction, 61(4), 1017-1022 (1999-09-24)

There is sufficient evidence to prove that tumor necrosis factor alpha (TNFalpha) modulates bovine corpus luteum (CL) function. Our previous study demonstrated that functional TNFalpha receptors are present on luteal cells in bovine CL throughout the estrous cycle. The purpose

Efficient generation of dopamine neurons from human embryonic stem cells.

Chang-Hwan Park et al.

Methods in molecular biology (Clifton, N.J.), 407, 311-322 (2008-05-06)

In this chapter, we introduce a co-culture protocol for human embryonic stem (hES) cell differentiation in which dopamine (DA) neurons with midbrain-specific markers are efficiently derived. Human ES cells on a feeder layer of stromal cells are induced to differentiate

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