A8792
Anti-Human IgG (whole molecule)−Peroxidase antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Synonym(s):
HRP Rabbit Anti-Human IgG (whole molecule)
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About This Item
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
DB, ELISA (d), IHC (p)
Citations:
29
biological source
rabbit
conjugate
peroxidase conjugate
antibody form
IgG fraction of antiserum
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
human
technique(s)
direct ELISA: 1:40,000, dot blot: 1:80,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Related Categories
General description
Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. IgG is usually found as a monomer. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. About 70 percent of the total immunoglobulin consists of IgG.
Immunogen
purified human IgG
Application
Anti-Human IgG (whole molecule)-Peroxidase antibody produced in rabbit has been used in:
enzyme-linked immunosorbent assay (ELISA)
western blotting
microarray analysis
enzyme-linked immunosorbent assay (ELISA)
western blotting
microarray analysis
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
Western Blotting (1 paper)
Enzyme-linked immunosorbent assay (1 paper)
Western Blotting (1 paper)
Biochem/physiol Actions
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.
Rabbit Anti-Human IgG (whole molecule)-Peroxidase antibody binds to human IgG.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT
Preparation Note
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Jenny Calow et al.
mAbs, 8(8), 1498-1511 (2016-10-30)
Antibody glycosylation is a key parameter in the optimization of antibody therapeutics. Here, we describe the production of the anti-cancer monoclonal antibody rituximab in the unicellular ciliate, Tetrahymena thermophila. The resulting antibody demonstrated enhanced antibody-dependent cell-mediated cytotoxicity, which we attribute
Rajika Lasanthi Dewasurendra et al.
BMC infectious diseases, 17(1), 49-49 (2017-01-11)
Sri Lanka achieved the WHO certificate as a malaria free country in September 2016, thus monitoring of malaria transmission using sensitive and effective tools is an important need. Use of age-specific antibody prevalence as a serological tool to predict transmission
Identification and clinical significance of an elevated level of serum aminoacylase-1 autoantibody in patients with hepatitis B virus-related liver cirrhosis
He X, et al.
Molecular Medicine Reports, 14(5), 4255-4262 (2016)
Rajika L Dewasurendra et al.
Malaria journal, 17(1), 473-473 (2018-12-19)
Antibodies against the merozoite surface protein 1-19 (MSP1-19) and the apical membrane antigen 1 (AMA1) of the malaria parasite (Plasmodium vivax) are proven to be important in protection against clinical disease. Differences in the production/maintenance of antibodies may be due
Carlos Sánchez-Ovejero et al.
PLoS neglected tropical diseases, 14(11), e0008892-e0008892 (2020-12-01)
Cystic echinococcosis (CE) is a neglected zoonotic disease caused by Echinococcus granulosus sensu lato. Diagnosis and monitoring of CE rely primarily on imaging while serology is used as a confirmatory test. However, imaging is not always conclusive and currently available
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