E8897
Monoclonal Anti-Ezrin antibody produced in mouse
clone 3C12, ascites fluid
Synonym(s):
Anti-CVIL, Anti-CVL, Anti-HEL-S-105, Anti-VIL2
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About This Item
Conjugate:
unconjugated
Clone:
3C12, monoclonal
Application:
immunocytochemistry
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
immunoprecipitation (IP)
microarray
western blot
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
immunoprecipitation (IP)
microarray
western blot
Species reactivity:
human, mouse, hamster, monkey, kangaroo rat, bovine
Citations:
65
Technique(s):
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1:4,000 using cultured human fibroblast cell line extract
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1:4,000 using cultured human fibroblast cell line extract
Uniprot accession no.:
Product Name
Monoclonal Anti-Ezrin antibody produced in mouse, clone 3C12, ascites fluid
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
3C12, monoclonal
mol wt
antigen 80 kDa
contains
15 mM sodium azide
species reactivity
human, mouse, hamster, monkey, kangaroo rat, bovine
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1:4,000 using cultured human fibroblast cell line extract
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... EZR(7430)
mouse ... Ezr(22350)
rat ... Ezr(54319)
Application
The antibody was used for detection of Ezrin by immunostaining, immunoblotting in human bladder cancer cells , immunohistochemistry, immunoprecipitation in kidney and colon adenocarcinoma cells and electron microscopy in astrocytes
Biochem/physiol Actions
Ezrin links the cytoskeleton to the plasma membrane, based on its amino acid sequence homologies with other cytoskeletal proteins. It regulates cell growth, migration, and cell adhesion. Ezrin is also implicated in cancer cell invasion and metastasis. The proteins of the ezrin family serve as tyrosine kinase phosphorylation substrates. They exhibit a growth factor receptor-specific pattern of phosphorylation. Ezrin is phosphorylated by epidermal growth factor receptor and platelet-derived growth factor receptor.
Monoclonal Anti-Ezrin recognizes ezrin (80 kDa). Cross-reactivity is observed with human, monkey, bovine, rat, kangaroo, hamster, and mouse
The ezrin/radixin/moesin (ERM) family regulates the dynamic cell membrane functions such as endocytosis, exocytosis, and transmembrane and other cortical signalling pathways. Ezrin (molecular weight 80 kDa) interacts with transmembrane proteins and F-actin and mediate important signalling pathways including RhoA and Hedgehog signalling.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Ezrin is a member of the best studied member of the ERM family of proteins. The ERM family regulates the dynamic cell membrane functions such as endocytosis, exocytosis, and transmembrane and other cortical signalling pathways. Ezrin (molecular weight 80 kDa) interacts with transmembrane proteins and F-actin and mediate important signalling pathways including RhoA and Hedgehog signalling. Ezrin is also present in the microvillus surface of acid-secreting parietal cells of the gastric glands, where it presents as a family of isoelectric variants. By immunoelectron microscopy of human choriocarcinoma cells, ezrin is enriched just inside the plasma membrane of microvilli and less abundant on more planar aspects of the membrane.
Monoclonal Anti-Ezrin (mouse IgG1 isotype) is derived from the 3C12 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Ezrin is present in microvilli, placental microvilli, microspikes, and membrane ruffles of cultured cells. It is also found in the growth cones of cultured neurons and the marginal band of avian erythrocytes. Ezrin comprises a (4.1 protein, ezrin, radixin and moesin) FERM domain, an α-helical linker region and a C-terminal domain. The amino-terminal domain of ezrin is localized to membranes and the carboxy-terminal part, containing the α-helical domain, colocalizes with actin filaments. Ezrin gene is mapped to the human chromosome location 6q25.3.
Immunogen
carboxy-terminal section of recombinant human ezrin (amino acids 362-585).
Physical form
Monoclonal Anti-Ezrin is provided as ascites fluid with 15 mM sodium azide as a preservative.
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
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Storage Class
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Subcellular distribution and expression of cofilin and ezrin in human colon adenocarcinoma cell lines with different metastatic potential
Nowak D et al
European Journal of Histochemistry, 52, e14-e14 (2010)
A Derouiche et al.
Glia, 36(3), 330-341 (2001-12-18)
Astrocytes extend thin lamellate processes in the neuropil, in particular around synapses, where they can modulate synaptic function or mediate glial-neuronal communication. Previous studies have shown that these lamellate perisynaptic processes change their shape in response to neuronal activity, but
Haydar Çelik et al.
The Journal of biological chemistry, 291(25), 13257-13270 (2016-05-04)
Ezrin is a member of the ERM (ezrin/radixin/moesin) family of proteins that links cortical cytoskeleton to the plasma membrane. High expression of ezrin correlates with poor prognosis and metastasis in osteosarcoma. In this study, to uncover specific cellular responses evoked
Aleksandar Ivetic et al.
Immunology, 112(2), 165-176 (2004-05-19)
The ezrin/radixin/moesin (ERM) family of actin-binding proteins act both as linkers between the actin cytoskeleton and plasma membrane proteins and as signal transducers in responses involving cytoskeletal remodelling. The Rho family of GTPases also regulate cytoskeletal organisation, and several molecular
Kiyoharu J Miyagishima et al.
Stem cells translational medicine, 5(11), 1562-1574 (2016-07-13)
: Induced pluripotent stem cells (iPSCs) can be efficiently differentiated into retinal pigment epithelium (RPE), offering the possibility of autologous cell replacement therapy for retinal degeneration stemming from RPE loss. The generation and maintenance of epithelial apical-basolateral polarity is fundamental
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