G6637
β-Galactose Dehydrogenase from Pseudomonas fluorescens
recombinant, expressed in E. coli, ammonium sulfate suspension, ≥50 units/mg protein (biuret)
Synonym(s):
D-Galactose:NAD+ 1-oxidoreductase
Sign In to View Organizational & Contract Pricing.
Select a Size
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-837-4
MDL number:
Specific activity:
≥50 units/mg protein (biuret)
Assay:
0.5—2.0 mg protein/mL (biuret)
Biological source:
Pseudomonas fluorescens
Recombinant:
expressed in E. coli
biological source
Pseudomonas fluorescens
Quality Level
recombinant
expressed in E. coli
assay
0.5—2.0 mg protein/mL (biuret)
form
ammonium sulfate suspension
specific activity
≥50 units/mg protein (biuret)
color
white
suitability
suitable for enzyme test
application(s)
life science and biopharma
shipped in
wet ice
storage temp.
2-8°C
Gene Information
Pseudomonas fluorescens ... gdh(533113295)
Application
β-Galactose Dehydrogenase from Pseudomonas fluorescens has been used for competitive inhibition in lectin histochemistry. It has also been used to measure the hydrolysis activity of Haloferax alicantei β-galactosidase on different disaccharides.
Biochem/physiol Actions
β-galactose dehydrogenase catalyzes the oxidation of β-D-galactose to D-galactono-gammalactone.
Physical form
Suspension in 3.2 M (NH4)2SO4, pH approx. 6.0
Other Notes
One unit will convert 1.0 μmole of D-galactose to D-galactonate per min at pH 8.6 at 25 °C.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Takahiro Mieda et al.
Plant & cell physiology, 45(9), 1271-1279 (2004-10-29)
We have studied the enzymological properties of L-galactose dehydrogenase (l-GalDH), a key enzyme in the biosynthetic pathway of l-ascorbate (AsA) in plants. L-GalDH was purified approximately 560-fold from spinach leaves. The enzyme was a homodimer with a subunit mass of
Optimizing the enzymatic determination of galactose in the culture medium of rat liver and HepG2 cell spheroids.
Jinsheng Xu et al.
Analytical biochemistry, 311(2), 179-181 (2002-12-10)
T E Curey et al.
Analytical biochemistry, 293(2), 178-184 (2001-06-12)
We report the development of a sensor for rapidly and simultaneously measuring multiple sugars in aqueous samples. In this strategy, enzyme-based assays are localized within an array of individually addressable sites on a micromachined silicon chip. Microspheres derivatized with monosaccharide-specific
Sensory properties and taste compounds of fermented milk produced by Lactococcus lactis and Streptococcus thermophius
M. Omaea et al.
Food Sci. Technol., 14, 183-189 (2008)
D Peltzer et al.
Free radical research, 31 Suppl, S181-S185 (2000-02-29)
Green and white variegated leaves of Coleus blumei, Benth. were separated into albino and green sections and used to determine the distribution of vitamin C and L-galactose dehydrogenase activity, an enzyme supposed to be involved in ascorbate metabolism, in heterotrophic
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service