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Merck

L2500

L-Lactic Dehydrogenase from rabbit muscle

Type II, ammonium sulfate suspension, 800-1,200 units/mg protein

Synonym(s):

Lactate, (S)-Lactate: NAD+ oxidoreductase, L-LDH, LAD, LD

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About This Item

CAS Number:
9001-60-9
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-617-8
MDL number:
MFCD00131466
EC Number:
1.1.1.27 (BRENDA, IUBMB)
Specific activity:
800-1,200 units/mg protein

Key Documents

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type

Type II

form

ammonium sulfate suspension

specific activity

800-1,200 units/mg protein

mol wt

140 kDa

foreign activity

pyruvate kinase, myokinase, malic dehydrogenase, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase and α-glycerophosphate dehydrogenase ≤0.01%

shipped in

wet ice

storage temp.

2-8°C

Quality Level

200

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Application

L-Lactic Dehydrogenase from rabbit muscle has been used:
  • for protein-binding measurement
  • to detect the action of oxaloacetate decarboxylase
  • to determine serum L- and D-lactate

Biochem/physiol Actions

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.
Lactic dehydrogenase is responsible for the conversion of pyruvate to lactate in the fermentative metabolism.

Analysis Note

Protein determined by biuret.

General description

Lactic Dehydrogenase (LDH) has a total molecular weight of 140 kDa and is composed of 4 subunits which are designated M subunit (muscle) and H subunit (heart). These subunits may be mixed in any of 5 combinations (M4, M3H1, M2H2, MH3, and H4). Skeletal muscle contains LDH that is predominately M4 with some small amounts of M3H and traces of H2H2. The H and M subunits are quite similar in molecular weight, but differ substantially in amino acid composition. Rabbit muscle LDH dissociates into dimeric species (MW = ~70 kDa) in acetate-chloride at pH 5.0, the dissociation is reversible. Biochemistry, 13, 3527-3531 (1974). Oxidizes glyoxylate and lactate.
Isoelectric point: 8.4-8.6
Optimal pH : 7.5 .

Other Notes

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

Physical form

Crystalline suspension in 3.2 M (NH4)2SO4 solution, pH 6.0

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 1

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000528315
0000528308
0000528312
0000528311
0000528315

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Duration of time that beef cattle are fed a high-grain diet affects the recovery from a bout of ruminal acidosis: Short-chain fatty acid and lactate absorption, saliva production, and blood metabolites
Schwaiger T, et al.
Journal of Animal Science, 91(12), 5743-5753 (2013)
Nutritional Biochemistry, 233-233 (1999)
Autofluorescence from NADH conformations associated with different metabolic pathways monitored using nanosecond-gated spectroscopy and spectral phasor analysis
Maltas J, et al.
Analytical Chemistry, 87(10), 5117-5124 (2015)
Anaerobic growth of Escherichia coli on D-tartrate depends on the fumarate carrier DcuB and fumarase, rather than the L-tartrate carrier TtdT and L-tartrate dehydratase
Kim OB, et al.
Archives of Microbiology, 188(6), 583-589 (2007)
Jingxuan Cui et al.
Metabolic engineering communications, 10, e00122-e00122 (2020-02-07)
Thermoanaerobacterium saccharolyticum is an anaerobic thermophile that can ferment hemicellulose to produce biofuels, such as ethanol. It has been engineered to produce ethanol at high yield and titer. T. saccharolyticum uses the Embden-Meyerhof-Parnas (EMP) pathway for glycolysis. However, the genes and
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