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About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder
form
lyophilized powder
potency
<20 μg/mL agglutination activity
composition
Protein, 100% biuret
impurities
salt, essentially free
storage temp.
2-8°C
Quality Level
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Biochem/physiol Actions
WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.
Preparation Note
Conjugates are prepared from affinity purified lectin.
Analysis Note
Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hour incubation at 25 °C.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Julie Beneteau et al.
Plant physiology, 153(3), 1345-1361 (2010-05-06)
Phloem Protein2 (PP2) is a component of the phloem protein bodies found in sieve elements. We describe here the lectin properties of the Arabidopsis (Arabidopsis thaliana) PP2-A1. Using a recombinant protein produced in Escherichia coli, we demonstrated binding to N-acetylglucosamine
André A Neves et al.
Endoscopy, 50(6), 618-625 (2018-01-18)
Endoscopic surveillance for Barrett's esophagus (BE) is limited by long procedure times and sampling error. Near-infrared (NIR) fluorescence imaging minimizes tissue autofluorescence and optical scattering. We assessed the feasibility of a topically applied NIR dye-labeled lectin for the detection of
Saurabh Raj et al.
Biomedical optics express, 3(4), 753-763 (2012-05-11)
Two microparticles were biochemically attached to a red blood cell at diametrically opposite parts and held by optical traps allowing to impose deformations. The cell deformation was monitored from the microscopy images. Raman spectra of the cell under tunable deformations
Ilaria Volpi et al.
Open biology, 11(10), 210121-210121 (2021-10-27)
Treslin/Ticrr is required for the initiation of DNA replication and binds to MTBP (Mdm2 Binding Protein). Here, we show that in Xenopus egg extract, MTBP forms an elongated tetramer with Treslin containing two molecules of each protein. Immunodepletion and add-back
Qian Li et al.
Foods (Basel, Switzerland), 10(12) (2021-12-25)
Paeonol can effectively inhibit Aspergillus flavus (A. flavus) via damaging cell walls. In this work, paeonol treatment remarkably destroyed both the outer amorphous layer and the inner fibrous layer of cell walls. Furthermore, FT-IR and XPS characterization showed that OH
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