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Purospher® STAR NH2 (5 µm) HPLC Columns

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Purospher® STAR NH2 HPLC ColumnsMerck offers a broad range of HPLC columns that are fully compliant with USP (United States Pharmacopoeia) specifications. The L8 USP column is described as an essentially monomolecular layer of aminopropylsilane (NH2), chemically bonded to a totally porous silica gel support, 3 to 10 µm in diameter. These criteria are best met by high-quality L8 USP columns, such as Purospher® STAR NH2 (5 µm).

Excellent for Carbohydrate Analysis

Purospher® STAR NH2 (5 µm) HPLC columns are based on high-purity silica with an amino-bonded phase. The columns are primarily designed for carbohydrate analysis, with a typical mobile phase consisting of acetonitrile and water. The benefits of these columns include excellent separation efficiency, and the absence of metallic impurities, resulting in consistently symmetrical peaks. Purospher® STAR NH2 columns are also suitable for use in the normal-phase retention mode.

Feature and Benefits

  • Classified as L8 USP column
  • Optimized for carbohydrate analysis
  • Excellent separation efficiency as measured by plate count
  • Absence of metal impurities for consistently symmetrical peaks
  • Extended column lifetime

Application Example

Separation of pharmaceuticals with non-buffered eluent

Specifications of Purospher® STAR NH2 (5 µm)

Sorbent Characteristics
High-purity silica gel with NH2 (amino) modification
Metal Content
Na, Ca, Mg, Al: 1 ppm; Fe: 3 ppm
Particle Shape
Spherical
Particle Size
5 μm
Pore Size 120 A (12 nm)
Pore Volume 1.1 ml/g
Spec. Surface Area 330 m²/g
Carbon Load 3.5 %
Coverage of the Surface 3 µmol/m²
pH Range pH 2-7.5
Efficiency > 50.000 N/m
Shipping Eluent n-Heptane
 
 
 
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