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Merck

05-644

Anti-CAR Antibody, clone RmcB

clone RmcB, Upstate®, from mouse

Synonym(s):

46 kD coxsackievirus and adenovirus receptor (CAR) protein, CVB3 binding protein, CVB3-binding protein, Coxsackievirus B-adenovirus receptor, coxsackie virus B receptor, coxsackie virus and adenovirus receptor

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Product Name

Anti-CAR Antibody, clone RmcB, clone RmcB, Upstate®, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

RmcB, monoclonal

species reactivity

human, rat, mouse

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_001338

UniProt accession no.

P78310

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... CXADR(1525)

Analysis Note

Evaluated by Western Blot on mouse small intestine lysates.

Western Blot Analysis:
1:500 dilution of this antibody detected Car on 10 µg of mouse small intestine lysates.

Application

Anti-CAR Antibody, clone RmcB detects level of CAR & has been published & validated for use in IC & WB.
Immunocytochemistry:
1-2 μg/mL of a previous lot showed positive immunostaining for CAR in HeLa cells, hCAR transfected CHO cells, but not in untransfected CHO cells fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton-X.

Biochem/physiol Actions

Recognizes CAR at MW ~46 kDa.

General description

The coxsackievirus and adenovirus receptor (CAR) mediates cell attachment and infection by coxsackie B viruses and by a number of adenoviruses. CAR also mediates homotypic intercellular interactions. In polarized epithelial cells, CAR is closely associated with the tight junction, where it contributes to the barrier to paracellular flow of solutes and macromolecules. CAR′s biological roles are not well defined, but emerging evidence suggests that it may function during embryonic development and in regulating cell proliferation.
~46 kDa

Immunogen

Native human coxsackie virus-adenovirus receptor protein.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified
Purified mouse monoclonal IgG1 in buffer containing 0.1M Tris-Glycine, 0.15M NaCl and 0.05% Sodium Azide, pH 7.4.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A monoclonal antibody specific for the cellular receptor for the group B coxsackieviruses.
Hsu KH, Lonberg-Holm K, Alstein B, Crowell RL.
Journal of virology, 62, 1647-1652 (1988)
Estrella Lopez-Gordo et al.
Journal of virology, 91(12) (2017-04-07)
Human adenoviral serotype 5 (HAdV-5) vectors have predominantly hepatic tropism when delivered intravascularly, resulting in immune activation and toxicity. Coagulation factor X (FX) binding to HAdV-5 mediates liver transduction and provides protection from virion neutralization in mice. FX is dispensable
Adenovirus-induced thrombocytopenia: the role of von Willebrand factor and P-selectin in mediating accelerated platelet clearance.
Othman, M; Labelle, A; Mazzetti, I; Elbatarny, HS; Lillicrap, D
Blood null
Y Watanabe et al.
Cancer gene therapy, 19(11), 767-772 (2012-09-08)
Replication-selective oncolytic viruses are being developed for human cancer therapy. We previously developed an attenuated adenovirus (OBP-301, Telomelysin), in which the human telomerase reverse transcriptase promoter element drives expression of E1A and E1B genes linked with an internal ribosome entry
William C Adams et al.
The Journal of general virology, 90(Pt 7), 1600-1610 (2009-03-14)
The coxsackievirus-adenovirus receptor (CAR) is the described primary receptor for adenovirus serotype 5 (Ad5), a common human pathogen that has been exploited as a viral vector for gene therapy and vaccination. This study showed that monocytes and dendritic cells (DCs)
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Global Trade Item Number

SKUGTIN
05-64404053252621970

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