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Merck

481001

Anti-Neutrophil Elastase Rabbit pAb

liquid, Calbiochem®

Synonym(s):

Neutrophil elastase antibody, Rabbit monoclonal antibody

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About This Item

NACRES:
NA.43
UNSPSC Code:
12352203

Product Name

Anti-Neutrophil Elastase Rabbit pAb, liquid, Calbiochem®

biological source

rabbit

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

dilution

(ELISA (1:1000)
Immunoblotting
Immunoelectrophoresis
Immunoprecipitation )

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... ELANE(1991)

Immunogen

Human
human neutrophil elastase

Other Notes

Single arc by immunoelectrophoresis against neutrophil granule extract and human neutrophil elastase. This antibody has also been reported to work for immunoblotting and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, pH 7.5.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Application

ELISA (≥ 1:1000)

Immunoblotting (see comments)

Immunoelectrophoresis (see comments)

Immunoprecipitation (see comments)

Disclaimer

Toxicity: Standard Handling (A)

General description

Ammonium sulfate precipitated rabbit polyclonal antibody. Recognizes human neutrophil elastase.
Recognizes the neutrophil elastase protein.
This Anti-Neutrophil Elastase Rabbit pAb is validated for use in ELISA, Immunoblotting, Immunoelectrophoresis, Immunoprecipitation for the detection of Neutrophil Elastase.

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Avinash Khandagale et al.
Journal of leukocyte biology, 104(6), 1199-1213 (2018-08-15)
Mutations in the gene JAGN1 were recently discovered in patients with severe congenital neutropenia (SCN). Neutrophils release neutrophil extracellular traps (NETs) consisting of decondensed chromatin decorated with various granular proteins such as neutrophil elastase and myeloperoxidase (MPO) to combat microbial
Elaine F Kenny et al.
eLife, 6 (2017-06-03)
Neutrophils release neutrophil extracellular traps (NETs) which ensnare pathogens and have pathogenic functions in diverse diseases. We examined the NETosis pathways induced by five stimuli; PMA, the calcium ionophore A23187, nigericin, Candida albicans and Group B Streptococcus. We studied NET
Dae-goon Yoo et al.
Journal of immunology (Baltimore, Md. : 1950), 192(10), 4728-4738 (2014-04-18)
Cystic fibrosis (CF) airways are characterized by bacterial infections, excess mucus production, and robust neutrophil recruitment. The main CF airway pathogen is Pseudomonas aeruginosa. Neutrophils are not capable of clearing the infection. Neutrophil primary granule components, myeloperoxidase (MPO) and human
Volker Brinkmann et al.
Frontiers in immunology, 3, 413-413 (2013-01-15)
Neutrophil Extracellular Traps (NETs) consist of decondensed chromatin studded with granular and some cytoplasmic proteins. They are formed by activated neutrophil granulocytes, also called polymorphonuclear leukocytes (PMN) as the result of an active cell death program, named NETosis. NETosis can
Frauke Pedersen et al.
Respiratory medicine, 109(10), 1360-1362 (2015-09-01)
Chronic obstructive pulmonary disease (COPD) is characterized by neutrophilic airway inflammation. Neutrophil extracellular trap (NET) formation - a meshwork of neutrophil DNA components and neutrophil enzymes are involved in innate immunity and inflammation. Little is known about the presence of
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