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H3884

Hyaluronidase from bovine testes

Type IV-S, lyophilized powder (essentially salt-free), 750-3000 units/mg solid

Synonym(s):

Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase

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About This Item

CAS Number:
37326-33-3
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-464-3
MDL number:
MFCD00131351
EC Number:
3.2.1.35 (BRENDA, IUBMB)

Product Name

Hyaluronidase from bovine testes, Type IV-S, lyophilized powder (essentially salt-free), 750-3000 units/mg solid

biological source

bovine testis

conjugate

conjugate (Glucosaminoglycan)

type

Type IV-S

form

lyophilized powder (essentially salt-free)

specific activity

750-3000 units/mg solid

mol wt

~55 kDa (four subunits of 14 kDa each)

UniProt accession no.

Q5E985
Q8SQG8

application(s)

diagnostic assay manufacturing

relevant disease(s)

cancer

storage temp.

−20°C

Quality Level

200

Gene Information

cow ... HYAL1(515397), HYAL2(281838)

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Related Categories

Biochem/physiol Actions

Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.
These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Other Notes

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000517507
0000517507
0000497718
0000497722
0000497718

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  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

  2. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. Can stock solutions of Product H3884, Hyaluronidase from bovine testes, be stored frozen after reconstitution?

    Although Sigma-Aldrich has not tested the stability of stock solutions of Product H3884, Hyaluronidase from bovine testes, several publications cite storage of solutions of H3884 at -20 °C:1.  Campbell, Keith H.S, "Transgenic Sheep form Cultured Cells", from Transgenesis Techniques: Principles and Protocols, Methods Mol. Biol., 180, 289-301 (2002).  A 1500 IU/mL stock solution was stored in 20 mL aliquots at -20 °C.2.  Marschall, S. and de Angelis, M.H., "In Vitro Fertilization/Cryopreservation", from Transgenic Mouse: Methods and Protocols, Methods in Molecular Biology, 209, pp. 35-50. Humana Press (2003).  1 mg/mL stock solutions in M2 medium or water, sterile-filtered (0.22 mm filter) can be stored in aliquots at -20 °C.

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