MAK037
NAD/NADH Quantitation Kit
sufficient for 100 colorimetric tests
Synonym(s):
NAD/NADH Assay Kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
eCl@ss:
32161000
detection method
colorimetric
usage
sufficient for 100 colorimetric tests
relevant disease(s)
cancer
storage temp.
−20°C
General description
Nicotinamide adenine dinucleotide (NAD) is an enzymatic cofactor involved in many redox reactions. NAD functions as an electron carrier, cycling between the oxidized (NAD) and reduced (NADH) forms. In addition to its role in redox reactions, NAD plays critical roles in ADP (adenosine diphosphate)-ribosylation reactions and as a substrate for sirtuins.
Application
NAD/NADH Quantitation Kit has been used to quantify NAD+/NADH influenced by SIRT1 (sirtuin 1) activity (associated with ischemia-reperfusion injury) in the presence of trimetazidine.
Suitable for the detection of NADH and NAD in animal tissues (liver, kidney etc.), cell culture (adherent or suspension cells), serum or urine.
Biochem/physiol Actions
The NAD/NADH Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides, NAD (nicotinamide adenine dinucleotide) and NADH, and their ratio without the requirement to purify NAD/NADH from samples. This assay is specific for NAD and NADH, and does not detect NADP or NADPH. NADt (NAD and NADH) or NADH are quantified in a colorimetric assay (450 nm).
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Storage Class
10 - Combustible liquids
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