N2640

4-Nitrophenyl phosphate disodium salt hexahydrate

Name: 4-Nitrophenyl phosphate disodium salt hexahydrate
Brand: SIGMA

chromogenic, tablet

Synonym(s):

p-Nitrophenyl phosphate disodium hexahydrate, Disodium 4-nitrophenyl phosphate hexahydrate, di-Sodium 4-nitrophenyl phosphate, pNPP disodium hexahydrate

Select a Size

All Photos(2)

About This Item

Linear Formula:

O₂NC₆H₄OP(O)(ONa)₂· 6H₂O

CAS Number:

333338-18-4

Molecular Weight:

371.14

NACRES:

NA.83

PubChem Substance ID:

24897580

UNSPSC Code:

12352204

EC Number:

224-246-5

MDL number:

MFCD00066288

Beilstein/REAXYS Number:

5324661

Product Name

4-Nitrophenyl phosphate disodium salt hexahydrate, tablet

InChI key

KAKKHKRHCKCAGH-UHFFFAOYSA-L

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

SMILES string

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

form

tablet

solubility

water: 1 tablet/3 mL, clear, colorless

storage temp.

−20°C

Quality Level

200

Looking for similar products? Visit Product Comparison Guide

Related Categories

Chemistry & Biochemicals

Phosphates

Protein Biology

Proteins & Enzymes

Detection Substrates & Enzymes

Application

4-Nitrophenyl phosphate disodium salt hexahydrate has been used in enzyme-linked immunosorbent assay (ELISA) as a substrate for streptavidin-Alkaline Phosphatase. It has also been used as a substrate in control assay for comparing 4-aminophenyl phosphate/diethanolamine (APP/DEA) assay for alkaline phosphatase.

Biochem/physiol Actions

4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP yields p-nitrophenol. pNPP is a common chromogenic substrate for alkaline phosphatase.

General description

Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.

Preparation Note

Contains 15 mg substrate per tablet

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

0000467468

0000439116

0000439125

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Vitamin A differentially regulates cytokine expression in respiratory epithelial and macrophage cell lines

Penkert RR, et al.

Cytokine, 91, 1-5 (2017)

A mastoparan-derived peptide has broad-spectrum antiviral activity against enveloped viruses.

Christopher J Sample et al.

Peptides, 48, 96-105 (2013-07-31)

Broad-spectrum antiviral drugs are urgently needed to treat individuals infected with new and re-emerging viruses, or with viruses that have developed resistance to antiviral therapies. Mammalian natural host defense peptides (mNHP) are short, usually cationic, peptides that have direct antimicrobial

Protein tyrosine phosphatase assays

Lorenz U

Current Protocols in Immunology, 93(1), 11-17 (2011)

Alkaline Phosphatase Assay Based on the Chromogenic Interaction of Diethanolamine with 4-Aminophenol

Sun J, et al.

Analytical Chemistry, 90(10), 6339-6345 (2018)

Roles of mast cells in the pathogenesis of inflammatory myopathy.

Masaya Yokota et al.

Arthritis research & therapy, 16(2), R72-R72 (2014-03-19)

In addition to the pivotal roles of mast cells in allergic diseases, recent data suggest that mast cells play crucial roles in a variety of autoimmune responses. However, their roles in the pathogenesis of autoimmune skeletal muscle diseases have not

View All Related Papers