N9389

4-Nitrophenyl phosphate disodium salt hexahydrate

Name: 4-Nitrophenyl phosphate disodium salt hexahydrate
Brand: SIGMA

chromogenic, tablet

Synonym(s):

p-Nitrophenyl phosphate disodium hexahydrate, Disodium 4-nitrophenyl phosphate hexahydrate, di-Sodium 4-nitrophenyl phosphate, pNPP disodium hexahydrate

Select a Size

All Photos(2)

About This Item

Linear Formula:

O₂NC₆H₄OP(O)(ONa)₂· 6H₂O

CAS Number:

333338-18-4

Molecular Weight:

371.14

NACRES:

NA.83

PubChem Substance ID:

24897898

UNSPSC Code:

12352204

EC Number:

224-246-5

MDL number:

MFCD00066288

Beilstein/REAXYS Number:

5324661

Product Name

4-Nitrophenyl phosphate disodium salt hexahydrate, tablet

InChI key

KAKKHKRHCKCAGH-UHFFFAOYSA-L

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

SMILES string

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

description

5 mg substrate per tablet

form

tablet

fluorescence

λ_ex 310 nm (pH 7.1)
λ_ex 399 nm (Reaction Product)

storage temp.

−20°C

Quality Level

300

Looking for similar products? Visit Product Comparison Guide

Related Categories

Chemistry & Biochemicals

Phosphates

Protein Biology

Proteins & Enzymes

Detection Substrates & Enzymes

Application

4-Nitrophenyl phosphate disodium salt hexahydrate has been used in:

enzyme-linked immunosorbent assay (ELISA) to visualize glycoprotein VI (GPVI) binding to collagen type I (Col I)
enzymatic color reaction for semi-quantitative measurement of allergen-specific IgE by ELISA
alkaline phosphatase assay

General description

Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

The majority of allergen-specific IgE in the blood of allergic patients does not originate from blood-derived B cells or plasma cells.

J Eckl-Dorna et al.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 42(9), 1347-1355 (2012-08-29)

The production of allergen-specific IgE antibodies is a hallmark of IgE-mediated allergy but the contribution of blood cells to allergen-specific IgE production in allergic patients has not been studied in detail. Aim of this study was the characterization of IgE-producing

The morphogenetically active polymer, inorganic polyphosphate complexed with GdCl3, as an inducer of hydroxyapatite formation in vitro.

Xiaohong Wang et al.

Biochemical pharmacology, 102, 97-106 (2016-01-06)

Inorganic polyphosphate (polyP) is a physiological polymer composed of tens to hundreds of phosphate units linked together via phosphoanhydride bonds. Here we compared the biological activity of polyP (chain length of 40 phosphate units), complexed with Gd(3+) (polyP·Gd), with the

Preserved bioactivity and tunable release of a SDF1-GPVI bi-specific protein using photo-crosslinked PEGda hydrogels.

Marianne K Schesny et al.

Biomaterials, 35(25), 7180-7187 (2014-05-31)

Chemokine-induced stem cell recruitment is a promising strategy for post myocardial infarction treatment. Injection of stromal cell-derived factor 1 (SDF1) has been shown to attract bone marrow-derived progenitor cells (BMPCs) from the blood that have the potential to differentiate into

Roles of mast cells in the pathogenesis of inflammatory myopathy.

Masaya Yokota et al.

Arthritis research & therapy, 16(2), R72-R72 (2014-03-19)

In addition to the pivotal roles of mast cells in allergic diseases, recent data suggest that mast cells play crucial roles in a variety of autoimmune responses. However, their roles in the pathogenesis of autoimmune skeletal muscle diseases have not

Gluten exacerbates IgA nephropathy in humanized mice through gliadin-CD89 interaction.

Christina Papista et al.

Kidney international, 88(2), 276-285 (2015-03-26)

IgA1 complexes containing deglycosylated IgA1, IgG autoantibodies, and a soluble form of the IgA receptor (sCD89), are hallmarks of IgA nephropathy (IgAN). Food antigens, notably gluten, are associated with increased mucosal response and IgAN onset, but their implication in the

View All Related Papers