Skip to Content
Merck

P5994

Phosphatase substrate

40 mg tablets

Synonym(s):

4-Nitrophenyl phosphate disodium salt hexahydrate, pNPP disodium salt hexahydrate

Sign In to View Organizational & Contract Pricing.

Select a Size

All Photos(2)

About This Item

Linear Formula:
O2NC6H4OP(O)(ONa)2 · 6H2O
CAS Number:
333338-18-4
Molecular Weight:
371.14
NACRES:
NA.54
PubChem Substance ID:
24898723
UNSPSC Code:
12352204
EC Number:
224-246-5
MDL number:
MFCD00066288

Product Name

Phosphatase substrate, 40 mg tablets

InChI key

KAKKHKRHCKCAGH-UHFFFAOYSA-L

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

SMILES string

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

form

tablet

concentration

36-44 mg/tablet

impurities

p-nitrophenol, essentially free

solubility

H2O: soluble, clear, colorless to yellow (1 tablet in 8 mL of water.)

storage temp.

−20°C

Quality Level

200

Looking for similar products? Visit Product Comparison Guide

Related Categories

Application

Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.
Phosphatase is a chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. It is been used for the colorimetric detection of CTB fusion proteins. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.
Phosphatase substrate has been used as a substrate in alkaline phosphatase assay in serum samples, bone marrow stromal cells and human mesenchymal stem cells (hMSC).
The substrate has been used to determine the alkaline phosphatase (ALP) activity in human osteoblast-like Saos-2 cell lysates. ALP acts as an early marker of osteoblast differentiation that is expressed just before matrix mineralization begins.

General description

Human phosphatase-substrate networks are mapped based on the literature and protein-protein interaction (PPI) database. Based on gene expression data, the possible network of phosphatase-substrate-kinase is predicted.

Other Notes

Substrate for the determination of acid and alkaline phosphatases.

Preparation Note

Contains 40 mg substrate per tablet
Dissolve tablets to the desired concentration in either of the following buffers: 0.1 M glycine, pH 10.4, with 1 mM MgCl2 and 1 mM ZnCl2 or 1 M diethanolamine, pH 9.8, with 0.5 mM MgCl2. Typically, a pNPP concentration of 1 mg/mL is used.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000512813
0000512813
0000480748
0000480748
SLCP2840

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Evaluation of antibacterial, angiogenic, and osteogenic activities of green synthesized gap-bridging copper-doped nanocomposite coatings
Huang D, et al.
International journal of nanomedicine, 12(11), 7483-7483 (2017)
Dissociation of bone resorption and bone formation in adult mice with a non-functional V-ATPase in osteoclasts leads to increased bone strength
Henriksen K, et al.
PLoS ONE, 6(11), e27482-e27482 (2011)
Tetsuya Harakuni et al.
Infection and immunity, 73(9), 5654-5665 (2005-08-23)
Noninvasive mucosal vaccines are attractive alternatives to parenteral vaccines. Although the conjugation of vaccine antigens with the B subunit of cholera toxin (CTB) is one of the most promising strategies for vaccine delivery to mucosal immune systems, the molecule cannot
Gemma Mestres et al.
Acta biomaterialia, 8(3), 1169-1179 (2011-12-14)
α-Tricalcium phosphate (α-TCP) is widely used as a reactant in calcium phosphate cements. This work aims at doping α-TCP with silicon with a twofold objective. On the one hand, to study the effect of Si addition on the stability and
Elucidating human phosphatase-substrate networks
Li X, et al.
Science Signaling, 6(275), rs10-rs10 (2013)
View All Related Papers

Related Content

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service