SAB4200544
Anti-Synaptophysin antibody, Mouse monoclonal
clone SVP-38, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-MRXSYP, Monoclonal Anti-SYP, Monoclonal Anti-Synaptophysin1, Monoclonal Anti-Synaptoporin, Monoclonal Anti-Syp1, Monoclonal Anti-p38
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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
SVP-38, monoclonal
Application:
IF, IHC, WB
Citations:
25
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
SVP-38, monoclonal
form
buffered aqueous solution
mol wt
antigen 38 kDa
species reactivity
rat, human
concentration
~1.0 mg/mL
technique(s)
immunohistochemistry: 10-20 μg/mL using formalin-fixed paraffin embedded rat cerebellum., indirect immunofluorescence: 10-20 μg/mL using B35 cells., western blot: 1-2 μg/mL using newborn rat brain extracts.
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... SYP(6855)
General description
Anti-Synaptophysin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma SVP-38 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synaptosome preparation from rat retina. Synaptophysin is a glycoprotein containing four transmembrane domains with both N and C termini on the cytoplasm face.
Synaptophysin (SYP) is encoded by the gene mapped to human chromosome Xp11.23-p11.22. The encoded protein has a molecular mass of 38kDa and is exclusively localized to the membrane of presynaptic vesicles.
Immunogen
synaptosome preparation from rat retina. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2). The antibody is purified from culture supernatant of hybridoma cells grown in a bioreactor.
Application
Anti-Synaptophysin antibody, Mouse monoclonal has been used in following immunochemical techniques:
- Immunoblotting
- Immunohistochemistry
- Immunofluorescence
Biochem/physiol Actions
Anti-Synaptophysin antibody, Mouse monoclonal recognizes rat, human, guinea-pig, and pig synaptophysin
Synaptophysin (SYP) facilitates neurotransmitter release and synaptic plasticity. It is also implicated in synaptic vesicle formation and exocytosis. Mutation in the gene increases the risk of susceptibility to schizophrenia. It is widely used as a potential marker to study the circulation of synapses in the brain and to discover the basic features of the life cycle of synaptic vesicles (SVs).
Synaptophysin has also been implicated in the recycling of synaptic vesicles by associating with dynamin I, a GTPase required for endocytosis.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
Instructions
Association between SYP with attention-deficit/hyperactivity disorder in Chinese Han subjects: differences among subtypes and genders.
Liu L
Psychiatry Research, 210, 308-314 (2013)
Regulation of synaptophysin degradation by mammalian homologues of seven in absentia
Wheeler TC, et al.
The Journal of Biological Chemistry, 277(12), 10273-10282 (2002)
Mikihito Shibata et al.
Nature, 598(7881), 489-494 (2021-10-03)
The similarities and differences between nervous systems of various species result from developmental constraints and specific adaptations1-4. Comparative analyses of the prefrontal cortex (PFC), a cerebral cortex region involved in higher-order cognition and complex social behaviours, have identified true and