T9822

Anti-Tubulin, Polyglutamylated antibody, Mouse monoclonal

Name: Anti-Tubulin, Polyglutamylated antibody, Mouse monoclonal
Brand: SIGMA

clone B3, purified from hybridoma cell culture

Synonym(s):

Anti-K-ALPHA-1

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD03792435

Product Name

Anti-Tubulin, Polyglutamylated antibody, Mouse monoclonal, clone B3, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

B3, monoclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

mouse, sea urchin (Lytechinus pictus and three others.), bovine, monkey, rabbit, human, rat, Oxyrrhis marina (dinoflagellate), chicken

technique(s)

immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using cytosolic fraction of rat brain extract

isotype

IgM

UniProt accession no.

P68363

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... TUBA4A(7277), TUBB(203068)
mouse ... Tuba1a(22142), Tubb2b(73710)
rat ... Tuba1a(64158)

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Western Blotting (1 paper)

Monoclonal Anti-Tubulin, Polyglutamylated antibody produced in mouse has been used in:

immunoprecipitation
immunohistochemistry
immunofluorescence

Monoclonal anti-tubulin, polyglutamylated antibody can be used in immunoblotting, immunocytochemistry and ELISA. It can also be used as primary antibodies (diluted 1:1000) in western blotting.

Biochem/physiol Actions

Microtubule plays an important role in DNA segregation.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Tubulin is a 100kD protein that is present in all cells as a heterodimer of two similar polypeptides α and β which assemble to form microtubules. Proper organization of microtubules is essential for several cellular functions like mitosis, meiosis, some forms of organellar movement, intracellular transport, flagellar movement and cytoskeletal functions. Monoclonal anti-tubulin, polyglutamylated antibody can be used as primary antibody in colocalization experiments. This antibody can also be used for studies involving the inhibition of flagellar motility in permeabilized sperm models. Mouse anti-tubulin, polyglutamylated antibody reacts specifically with the epitope present in the C-terminal region of α and β tubulins (glutamylated motif at amino acids 445-457 of α-tubulin).

Tubulin is a cellular colchicine-binding protein.

Immunogen

purified sea urchin (Lytechinus pictus) sperm axonemal proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Mitotic spindle assembly and chromosome segregation: refocusing on microtubule dynamics

Kline-Smith SL and Walczak CE

Molecular Cell, 15(3), 317-327 (2004)

A Mutation in CCDC50, a Gene Encoding an Effector of Epidermal Growth Factor-Mediated Cell Signaling, Causes Progressive Hearing Loss

Modamio-HoybjorS, et al.

American Journal of Human Genetics, 80(6), 1076-1089 (2007)

The carboxyl terminal mutational hotspot of the ciliary disease protein RPGRORF15 (retinitis pigmentosa GTPase regulator) is glutamylated in vivo.

Kollu N Rao et al.

Biology open, 5(4), 424-428 (2016-03-05)

Mutations inRPGR(ORF15)(retinitis pigmentosa GTPase regulator) are a major cause of inherited retinal degenerative diseases. RPGR(ORF15)(1152 residues) is a ciliary protein involved in regulating the composition and function of photoreceptor cilia. The mutational hotspot in RPGR(ORF15)is an unusual C-terminal domain encoded

Triggering regeneration and tackling apoptosis: a combinatorial approach to treating congenital muscular dystrophy type 1 A.

Jenny Yamauchi et al.

Human molecular genetics, 22(21), 4306-4317 (2013-06-19)

Merosin-deficient congenital muscular dystrophy type 1A (MDC1A) is an autosomal recessive disorder caused by mutations in the laminin-α2 gene (OMIM: 607855). Currently, no treatment other than palliative care exists for this disease. In our previous work, genetic interventions in the

Insight into tubulin regulation from a complex with colchicine and a stathmin-like domain

Ravelli RBG, et al.

Nature, 428(6979), 198-198 (2004)

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