Anti-Tau oligomeric Antibody, clone TOMA-2

Microtubule-associated protein tau (UniProt P10636; also known as Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau) is encoded by the MAPT (also known as TAU, MAPTL, MTBT1) gene (Gene ID 4137) in human. In Alzheimer s disease (AD) pathology, accumulation of the microtubule-associated protein tau takes place primarily in the neurons. Tau accumulates in both the somatodendritic and axonal domains of neurons. Tau also accumulates in the soma as neurofibrillary tangles (NFTs). Cell death and synaptic lesions occur independently of NFT formation, and research indicates that NFT formation alone is insufficient for neurodegeneration, suggesting that soluble tau aggregates may be the more toxic and pathologically significant tau species. Tau oligomers are neurotoxic when applied extracellularly to cultured neuronal cells, and tau oligomers (but not fibrils) induce neurodegeneration and synaptic and mitochondrial dysfunction in vivo. Moreover, researchers are able to use tau oligomers as a reliable biomarker to differentiate AD brains from age-matched non-AD brains. Clone TOMA-2 is one of the 13 hybridomas derived from mice immunized with in vitro generated recombinant oligomers. It detects tau oligomers predominantly located in the cell bodies and neuronal nuclei and exhibits very dense signal in the cell nuclei. It exhibits higher affinity for microglial tau aggregates and microglia co‐stained with TOMA2 have strong immunoreactivity and colocalization. It is reported to recognize tau aggregates of 75 and 150 kDa in cerebral cortex in Alzheimer s disease (AD), Dementia with Lewy Bodies (DLB), and progressive supranuclear palsy (PSP). This clone is suitable for use in Western Blotting, Immunofluorescence, Immunohistochemistry, ELISA, Proximity Ligation Assay, Filter Trap Assay, and Dot Blot applications. (Ref.: Ruan, Z., et al. (2021). Brain. 144(1); 288-309; Montalbano, M., et al. (2023). Brain Pathol. 33(1); e13112; Arar, S., et al. (2024). ACS Chem. Neurosci. 15(7); 1366-1377; Lo Casio, F., et al. (2025). Commun Biol. 8(1); 53).

Oligomers of recombinant human Tau.

Quality Control Testing

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .

ELISA: A representative lot detected Tau oligomers in ELISA (Jiang, L., et al. (2021). Mol Cell. 81(20):4209-4227.e12; Lo Casio, F., et al. (2025). Commun Biol. 8(1):53).

Immunofluorescence Analysis: A representative lot detected Tau oligomers in Immunofluorescence application (Jiang, L., et al. (2019). Acta Neuropathol. 137(2):259-277; Montalbano, M., et al. (2020). Nat Commun. 11(1):4305; Jiang, L., et al. (2021). Mol Cell. 81(20):4209-4227.e12; Montalbano, M., et al. (2023). Brain Pathol. 33(1):e13112; Lo Casio, F., et al. (2025). Commun Biol. 8(1):53).

Western Blotting Analysis: A representative lot detected Tau oligomers in Western Blotting application (Montalbano, M., et al. (2023). Brain Pathol. 33(1):e13112; Lo Casio, F., et al. (2025). Commun Biol. 8(1):53).

Dot A representative lot detected Tau oligomers in Dot Blot application (Blot:Ruan, Z., et al. (2021). Brain. 144(1):288-309).

Proximity Ligation Assay: A representative lot was used in Proximity Ligation Assay application (Montalbano, M., et al. (2020). Nat Commun. 11(1):4305).

Immunohistochemistry Application: A representative lot detected Tau oligomers in Immunohistochemistry application (Jiang, L., et al. (2019). Acta Neuropathol. 137(2):259-277; Montalbano, M., et al. (2020). Nat Commun. 11(1):4305; Montalbano, M., et al. (2020). Neurobiol Dis. 146:105130; Jiang, L., et al. (2021). Mol Cell. 81(20):4209-4227.e12; Montalbano, M., et al. (2023). Brain Pathol. 33(1):e13112; Lo Casio, F., et al. (2025). Commun Biol. 8(1):53).

Filter Trap Assay: A representative lot of this antibody detected tau oligomers in Filter Trap Assay (Montalbano, M., et al. (2020). Neurobiol Dis. 146:105130).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone TOMA-2 is a mouse monoclonal antibody that specifically detects Tau oligomers in Alzheimer′s disease (AD) brain.

Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.