239765
Cycloheximide
hFKBP12 inhibitor, solution
Synonym(s):
InSolution Cycloheximide
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About This Item
Empirical Formula (Hill Notation):
C15H23NO4
CAS Number:
Molecular Weight:
281.35
UNSPSC Code:
12352111
NACRES:
NA.77
MDL number:
Product Name
Cycloheximide, InSolution, 100 mg/mL in DMSO, Suitable for cell culture
SMILES string
N1C(=O)CC(CC1=O)C[C@@H](O)[C@@H]2C[C@H](C[C@@H](C2=O)C)C
InChI
1S/C15H23NO4/c1-8-3-9(2)15(20)11(4-8)12(17)5-10-6-13(18)16-14(19)7-10/h8-12,17H,3-7H2,1-2H3,(H,16,18,19)/t8-,9-,11-,12+/m0/s1
InChI key
YPHMISFOHDHNIV-FSZOTQKASA-N
form
solution
manufacturer/tradename
Calbiochem®
concentration
100 mg/mL in DMSO
solubility
DMSO: soluble
storage temp.
2-8°C
Quality Level
Related Categories
Disclaimer
Toxicity: Highly Toxic & Carcinogenic / Teratogenic (I)
Biochem/physiol Actions
Primary Target
hFKBP12
hFKBP12
General description
A convenient ready-to-use form of Cycloheximide (Cat. No. 239763). An anti-fungal antibiotic that inhibits protein synthesis in eukaryotes but not in prokaryotes. Interacts directly with the translocase enzyme, interfering with the translocation step. Also inhibits cell-free protein synthesis in eukaryotes. Competitively inhibits hFKBP12 (Ki = 3.4 µM). Triggers apoptosis in HL-60 cells, T-cell hybridomas, Burkitt′s lymphoma cells, and a variety of other cell types, including rodent macrophages. Induces DNA fragmentation in macrophages, but inhibits DNA cleavage in rat thymocytes treated with thapsigargin, methylprednisolone, and ionomycin. Rapidly destroyed in alkaline solutions.
Other Notes
Christner, C., et al. 1999. J. Med. Chem.42, 3615.
Lu, Q. and Mellgreen,R.L. 1996. Arch. Biochem. Biophys.334, 175.
Chow, S.C., et al. 1995. Exp. Cell Res.216, 149.
Waring, P. 1990. J. Biol. Chem.265, 14476.
Obrig, T.G., et al. 1971. J. Biol. Chem.246, 174.
Pestka, S. 1971. Annu. Rev. Microbiol.25, 487.
Lu, Q. and Mellgreen,R.L. 1996. Arch. Biochem. Biophys.334, 175.
Chow, S.C., et al. 1995. Exp. Cell Res.216, 149.
Waring, P. 1990. J. Biol. Chem.265, 14476.
Obrig, T.G., et al. 1971. J. Biol. Chem.246, 174.
Pestka, S. 1971. Annu. Rev. Microbiol.25, 487.
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Packaging
Packaged under inert gas
Preparation Note
Following initial use, aliquot and refrigerate (4°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Physical form
A 100 mg/ml solution of Cycloheximide (Cat. No. 239763 ) in DMSO (sterile-filtered).
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 3 Oral - Aquatic Chronic 3 - Muta. 2 - Repr. 1B
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
Certificates of Analysis (COA)
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