Product Name
Anti-HA-Peroxidase, High Affinity, from rat IgG1
biological source
rat
conjugate
peroxidase conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
clone 3F10, monoclonal
form
lyophilized (clear, colorless solution after reconstitution)
packaging
pkg of 25 U (25 μg)
manufacturer/tradename
Roche
isotype
IgG1
epitope sequence
YPYDVPDYA
storage temp.
2-8°C
Quality Level
Related Categories
Preparation Note
Add 1.0 ml double-distilled water for a final concentration of 25 U/mL.
Rehydrate for 10 min prior to use.
Rehydrate for 10 min prior to use.
Stabilizers: proteinaceous stabilizers
Working concentration: The working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
Working concentration: The working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
- Dot blot: 50 mU/ml
- ELISA: 25 mU/ml
- Western blot: 50 mU/ml
Application
- Use Anti-HA-Peroxidase, High Affinity for the detection of HA-tagged recombinant proteins using: ELISA
- Western blot
Biochem/physiol Actions
Anti-HA-Peroxidase, High Affinity (3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein.
This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).
This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).
General description
Anti-HA-Peroxidase, High Affinity is a monoclonal antibody to the HA-peptide (clone 3F10), conjugated to peroxidase.
The Anti-HA High Affinity antibody (clone 3F10) recognizes the same epitope as clone 12CA5, which was originally used to study how the immune system recognizes the influenza hemagglutinin protein, a surface glycoprotein required for infectivity of the human virus. However, the principal use of the Anti-HA antibody is the detection and purification of proteins whose encoding DNA sequences have been fused to the HA epitope sequence by recombinant techniques, that is epitope tagging. The ability to prepare such epitope-tagged proteins and locate them with the Anti-HA antibody in subsequent experiments has enabled researchers to determine:
- The size, cellular localization, and abundance of proteins produced by newly discovered genes.
- Post-translational modifications of proteins.
- The movement of proteins within cell membranes.
- The identity of proteins within functional protein complexes.
- The function of proteins that are unstable, difficult to purify, or share epitopes with a number of other proteins.
Immunogen
The epitope consists of amino acids 98-106 from the human influenza virus hemagglutinin protein.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Not finding the right product?
Try our Product Selector Tool.
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Regina Gratz et al.
The New phytologist, 225(1), 250-267 (2019-09-06)
The key basic helix-loop-helix (bHLH) transcription factor in iron (Fe) uptake, FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR (FIT), is controlled by multiple signaling pathways, important to adjust Fe acquisition to growth and environmental constraints. FIT protein exists in active and inactive
Ranran Wang et al.
Cancers, 12(6) (2020-06-25)
The epigenetic reader BRD4 binds acetylated histones and plays a central role in controlling cellular gene transcription and proliferation. Dysregulation of BRD4's activity has been implicated in the pathogenesis of a wide variety of cancers. While blocking BRD4 interaction with
Satoshi Hashimoto et al.
Scientific reports, 10(1), 3422-3422 (2020-02-27)
Ribosome stalling triggers the ribosome-associated quality control (RQC) pathway, which targets collided ribosomes and leads to subunit dissociation, followed by proteasomal degradation of the nascent peptide. In yeast, RQC is triggered by Hel2-dependent ubiquitination of uS10, followed by subunit dissociation
Lina Herhaus et al.
Nature communications, 4, 2519-2519 (2013-09-28)
SMAD transcription factors are key intracellular transducers of TGFβ cytokines. SMADs are tightly regulated to ensure balanced cellular responses to TGFβ signals. Ubiquitylation has a key role in regulating SMAD stability and activity. Several E3 ubiquitin ligases that regulate the
Alicja M Cygan et al.
mSphere, 5(1) (2020-02-23)
Toxoplasma gondii is a ubiquitous, intracellular protozoan that extensively modifies infected host cells through secreted effector proteins. Many such effectors must be translocated across the parasitophorous vacuole (PV), in which the parasites replicate, ultimately ending up in the host cytosol
Related Content
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service