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Merck

319112

Methylene Blue solution

0.05 wt. % in H2O

Synonym(s):

Ehrlich’s reagent III, Loeffler’s Methylene Blue

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About This Item

Empirical Formula (Hill Notation):
C16H18ClN3S
CAS Number:
61-73-4
Molecular Weight:
319.85
NACRES:
NA.47
PubChem Substance ID:
24858888
UNSPSC Code:
12171500
Colour Index Number:
52015
MDL number:
MFCD00012111
Beilstein/REAXYS Number:
3641570

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InChI

1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI key

CXKWCBBOMKCUKX-UHFFFAOYSA-M

form

liquid

concentration

0.05 wt. % in H2O

density

0.98 g/mL at 25 °C

λmax

665 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Application

Methylene Blue solution has been used to study structural alterations and p53 immune-expression in rats.

Biochem/physiol Actions

Methylene blue (MB) is a highly recognized histological dye. It plays a vital role in microbiology and pharmacology. It has been employed in staining living organisms and in methemoglobinemia treatment. MB exhibits maximum absorption at 664nm. Exposure to methylene blue leads to various health hazards such as increased heart rate, vomiting, shock, Heinz body formation, cyanosis, jaundice, quadriplegia and tissue necrosis in humans

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SHBS4972
SHBR7946
MKCK4473
MKCD3437
MKBX9824V

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Alexandru Grigoroiu et al.
Scientific reports, 10(1), 3947-3947 (2020-03-05)
Hyperspectral imaging (HSI) is being explored in endoscopy as a tool to extract biochemical information that may improve contrast for early cancer detection in the gastrointestinal tract. Motion artefacts during medical endoscopy have traditionally limited HSI application, however, recent developments
Microscopic structural alterations
and p53 immune-expression in adult
rat testis in response to intra-tunical versus
intra-parenchymal injection of methylene blue
Mazroa S A
International Journal of Clinical and Experimental Pathology, 9, 5346-5356 (2016)
Methylene blue in photodynamic therapy: From basic mechanisms to clinical applications.
Tardivo JP
Photodiagnosis and Photodynamic Therapy, 2, 175-191 (2005)
J How et al.
Gynecologic oncology, 137(3), 436-442 (2015-04-15)
With the debate over extent of lymphadenectomy in endometrial cancer, sentinel lymph node (SLN) mapping may provide a focused approach to evaluate the most relevant lymph nodes (LN) while minimizing the complications. We evaluated SLN mapping using filtered technetium(99), indocyanine
Bram Vivijs et al.
Applied and environmental microbiology, 80(19), 6054-6061 (2014-07-27)
Some members of the family Enterobacteriaceae ferment sugars via the mixed-acid fermentation pathway. This yields large amounts of acids, causing strong and sometimes even lethal acidification of the environment. Other family members employ the 2,3-butanediol fermentation pathway, which generates comparatively
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