G6795
Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal
clone GSN24, purified from hybridoma cell culture
Synonym(s):
Anti-GFP
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About This Item
NACRES:
NA.56
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
GSN24, monoclonal
Application:
WB
Technique(s):
western blot: 1-2 μg/mL using GFP fusion proteins expressed in extracts of transfected cells
Citations:
39
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
GSN24, monoclonal
form
buffered aqueous solution
mol wt
antigen 27 kDa
concentration
~2 mg/mL
technique(s)
western blot: 1-2 μg/mL using GFP fusion proteins expressed in extracts of transfected cells
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C, 2-8°C
target post-translational modification
unmodified
Quality Level
Application
Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal has been used in: western blotting, enzyme linked immunosorbent assay (ELISA), immunofluorescence labeling.
Biochem/physiol Actions
Green Fluorescent Protein (GFP) is a unique tool in cellular and molecular biology research to monitor gene expression and protein localization in living cells. In the jellyfish, A. victoria, GFP transduces the excitation energy resulting from emission of blue light of the photoprotein aequorin and reemits it as green light.
The antibody reacts specifically with GFP fusion proteins.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma GSN24 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2–8 °C for up to one month. For extended storage, store at –20 °C in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Solutions at working dilution should be discarded if not used within 12 hours.
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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