H7039
3-Hydroxy-3-methylglutaryl-CoA reductase human
solution
Synonym(s):
HMGCoA Reductase, HMGR
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assay
≥90% (SDS-PAGE)
Quality Level
form
solution
specific activity
2-8 units/mg protein
mol wt
~76 kDa (for the fusion protein)
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... HMGCR(3156)
General description
Consists of the HMG CoA reductase catalytic domain fused to GST protein.
Application
3-Hydroxy-3-methylglutaryl-CoA reductase human has been used:
- in anti-HMGCR enzyme linked immunosorbent assay (ELISA)
- as an antigen to measure anti-HMGCR antibodies by blot analysis
- in HMG-CoA reductase activity assays
- as an antigen to compare in-house antigen via sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE)/western blot
Biochem/physiol Actions
Hydroxy-3-methylglutaryl-CoA reductase catalyzes the committed step in cholesterol biosynthesis.
3-Hydroxy-3-methylglutaryl-CoA reductase/HMGCR serves as a rate limiting enzyme in mevalonate pathway. It also controls the oncogenic Hippo transducers TAZ/YAP. HMGCR also perform as a genetic modifier for Alzheimer′s disease (AD).
Physical form
Supplied as a solution containing 250 μg protein in 50 mM Tris pH 7.5, with 5 mM DTT, 1:200 Protease Inhibitor Cocktail (Catalog Number P8340) and 50% (w/v) glycerol.
Other Notes
One unit will convert 1.0 μmole of NADPH to NADP+ per minute at 37 °C.
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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HMG-CoAR expression in male breast cancer: relationship with hormone receptors, Hippo transducers and survival outcomes
Di BA, et al.
Scientific reports, 6, 35121-35121 (2016)
OP0009 Distinctive Immunofluorescence Pattern in Statin-Associated Autoimmune Myopathy with Anti-HMGCR Autoantibodies
Alvarado-Cardenas M, et al.
Annals of the Rheumatic Diseases, 75, 56-56 (2016)
ChemVassa: a new method for identifying small molecule hits in drug discovery
Moldover B, et al.
The Open Medicinal Chemistry Journal, 6, 29-29 (2012)
Analysis of autoantibodies to 3-hydroxy-3-methylglutaryl-coenzyme A reductase using different technologies
Musset L, et al.
Journal of immunology research, 2014 (2014)
Myopathy with anti-HMGCR antibodies Perimysium and myofiber pathology
Alshehri A, et al.
Neurology? neuroimmunology & neuroinflammation, 2(4), e124-e124 (2015)
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