M7167
M2 medium
With HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for mouse embryo cell culture
Synonym(s):
Cell Culture M2, Cell Culture Medium, M2 Medium Solution
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About This Item
Quality Level
sterility
sterile-filtered
form
liquid
storage condition
protect from light
technique(s)
cell culture | embryo: suitable
impurities
endotoxin, tested
components
HEPES: 5.42726 g/L
NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L
shipped in
ambient
storage temp.
2-8°C
Application
Recommended for manipulation of mouse embryos at ambient temperature. M2 and M16 Medium are common media for in vitro culture of preimplantation stage embryos. It is a modified Krebs-Ringer bicarbonate solution, which is very similar to Whitten′s Medium. M16 contains pyruvate and lactate as energy sources since preimplantation embryos do not utilize glucose efficiently.
M2 Medium is a further modification of M16 that substitutes HEPES buffer in place of some of the bicarbonate. M2 is used for collecting and handling embryos for prolonged periods outside a CO2 incubator.
M2 Medium is a further modification of M16 that substitutes HEPES buffer in place of some of the bicarbonate. M2 is used for collecting and handling embryos for prolonged periods outside a CO2 incubator.
Preparation Note
Supplement with 0.06 g/L potassium penicillin-G and 0.05 g/L streptomycin sulfate.
Analysis Note
Presence of particulates in solution will not affect product′s performance.
This product is tested for its ability to support the development of one-cell mouse embryos to expanded blastocysts. Minimum requirement is 80% development to blastocyst.
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
Instructions
Hongzheng Sun et al.
Biology of reproduction, 100(3), 601-617 (2018-09-29)
Methionine adenosyltransferase II (MAT2A) is essential to the synthesis of S-adenosylmethionine, a major methyl donor, from L-methionine and ATP. Upon fertilization, zygotic genome activation (ZGA) marks the period that transforms the genome from transcriptional quiescence to robust transcriptional activity. During
Maryse Lessard et al.
Scientific reports, 9(1), 13829-13829 (2019-09-27)
The paternal environment is thought to influence sperm quality and future progeny may also be impacted. We hypothesized that prenatal exposure to environmentally-relevant contaminants impairs male reproduction, altering embryo gene expression over multiple generations. Folic acid (FA) can improve sperm
Takaya Totsuka et al.
Scientific reports, 10(1), 6556-6556 (2020-04-18)
To study the functional differences between maternal and paternal genomes in mammalian development, embryos with only one parental genome are often used. Androgenetic embryos are produced by the removal of maternal chromosomes before or after fertilization by techniques that require