T2507
Triosephosphate Isomerase from baker′s yeast (S. cerevisiae)
Type I, ammonium sulfate suspension, ~10,000 units/mg protein
Synonym(s):
D-Glyceraldehyde-3-phosphate ketol-isomerase, TPI
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-774-2
MDL number:
Specific activity:
~10,000 units/mg protein
type
Type I
Quality Level
form
ammonium sulfate suspension
specific activity
~10,000 units/mg protein
mol wt
53 kDa by equilibrium centrifugation
foreign activity
pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase <0.01%
storage temp.
2-8°C
Application
Triosephosphate isomerase has been used in a study to assess differential expression of fourteen proteins of uveal melanoma. Triosephosphate isomerase has also been used in a study to investigate the use of sigmoid pH gradients in free-flow isoelectric focusing of human endothelial cell proteins.
Physical form
Crystalline suspension in 2.7 M (NH4)2SO4, 0.5 mM EDTA, pH 6.5
Analysis Note
Protein determined by biuret.
Other Notes
One unit will convert 1.0 μmole D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.
Since the assay of this enzyme seems to be sensitive to minor variables, packages are dispensed per mg protein rather than in units.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Instructions
Xiao Ma et al.
Electrophoresis, 33(9-10), 1349-1355 (2012-06-01)
Prefractionation of proteins enhances the resolution of proteome analysis of whole cells. Free-flow electrophoresis (FFE) provides a useful step in various prefractionation protocols, since matrix-free isoelectric focusing (FF-IEF) performed in this machine enables the enrichment of large, easily absorbable, sensitive
Annett Linge et al.
Investigative ophthalmology & visual science, 53(8), 4634-4643 (2012-05-10)
To compare the proteomic profiles of two categories of primary uveal melanoma tissue samples; those from patients who have subsequently developed metastatic disease and those who have not. Two-dimensional difference gel electrophoresis (2D DIGE) was performed on 25 uveal melanoma
Xiaochen He et al.
Journal of cellular physiology, 237(8), 3317-3327 (2022-05-28)
Hypertension is an important risk factor in the pathogenesis of diastolic dysfunction. Growing evidence indicates that glucose metabolism plays an essential role in diastolic dysfunction. TP53-induced glycolysis and apoptosis regulator (TIGAR) has been shown to regulate glucose metabolism and heart