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Merck

16-266

Protein G Agarose, Fast Flow

Protein G Agarose, Fast Flow suitable for medium and low pressure chromatography of IgG from mouse, sheep, and rabbit, and for immunoprecipitations.

Synonym(s):

Protein G resin

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About This Item

UNSPSC Code:
41116133
NACRES:
NA.56
eCl@ss:
32160801

Product Name

Protein G Agarose, Fast Flow, Protein G Agarose, Fast Flow suitable for medium and low pressure chromatography of IgG from mouse, sheep, and rabbit, and for immunoprecipitations.

form

liquid

manufacturer/tradename

Upstate®

technique(s)

affinity chromatography: suitable
immunoprecipitation (IP): suitable
western blot: suitable

shipped in

wet ice

Related Categories

Analysis Note

routinely evaluated by immunoprecipitation and purification

Application

Protein G Agarose, Fast Flow has been used in immunoprecipitation. It is also suitable for medium and low-pressure chromatography of IgG from mice, sheep, and rabbits.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Fast flow agarose comprises highly crosslinked agarose beads or gels that can tolerate high pressures compared to standard agarose bead resins. Thus, these resins are ideal for fast protein liquid chromatography (FPLC) purification methods. They are preferred for medium to large-scale antibody purification. This product contains protein G covalently coupled by cyanogen bromide to highly cross-linked 4% agarose beads. It is stable in all aqueous buffers used in protein G chromatography.

Physical form

water containing 20% ethanol

Preparation Note

1 year at 4°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk

WGK 1

flash_point_f

116.6 °F

flash_point_c

47 °C

Certificates of Analysis (COA)

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Boshi Wang et al.
EBioMedicine, 41, 320-332 (2019-02-13)
Hyper-activation of TGF-β signaling is critically involved in progression of hepatocellular carcinoma (HCC). However, the events that contribute to the dysregulation of TGF-β pathway in HCC, especially at the post-translational level, are not well understood. Associations of deubiquitinase POH1 with
Lijing Liu et al.
The Plant journal : for cell and molecular biology, 61(5), 893-903 (2009-12-18)
The ubiquitination proteasome pathway has been demonstrated to regulate all plant developmental and signaling processes. E3 ligase/substrate-specific interactions and ubiquitination play important roles in this pathway. However, due to technical limitations only a few instances of E3 ligase-substrate binding and
Carsten Juel
PloS one, 9(10), e110514-e110514 (2014-10-14)
Changes in ion distribution across skeletal muscle membranes during muscle activity affect excitability and may impair force development. These changes are counteracted by the Na,K-ATPase. Regulation of the Na,K-ATPase is therefore important for skeletal muscle function. The present study investigated
Jie Hu et al.
FEBS letters, 592(11), 1893-1904 (2018-05-22)
SAMHD1 inhibits Hepatitis B virus (HBV) replication by reducing the intracellular dNTP levels. However, how SAMHD1 phosphorylation is regulated to abrogate its restriction of HBV replication in hepatoma cells is poorly understood. Here, we show that HBV replication and SAMHD1
Carsten Juel et al.
Physiological reports, 3(8) (2015-08-25)
Potassium and sodium displacements across the skeletal muscle membrane during exercise may cause fatigue and are in part controlled by the Na,K-ATPase. Regulation of the Na,K-ATPase is therefore important for muscle functioning. We investigated the effect of oxidative stress (glutathionylation)
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Global Trade Item Number

SKUGTIN
16-26604053252325731

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