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Merck

556881

Ribonuclease Inhibitor, Human, Recombinant, E. coli

The Ribonuclease Inhibitor, Human, Recombinant, E. coli controls the biological activity of Ribonuclease. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.

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About This Item

UNSPSC Code:
12352200

Key Documents

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Product Name

Ribonuclease Inhibitor, Human, Recombinant, E. coli, The Ribonuclease Inhibitor, Human, Recombinant, E. coli controls the biological activity of Ribonuclease. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.

form

liquid

specific activity

≥20,000 units/mL

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

foreign activity

Endonuclease and RNase, none detected

shipped in

wet ice

storage temp.

−20°C

Quality Level

100

Biochem/physiol Actions

Cell permeable: no
Primary Target
inactivates RNase
Product does not compete with ATP.
Reversible: no

Disclaimer

Toxicity: Standard Handling (A)

General description

Recombinant, human ribonuclease inhibitor expressed in E. coli. Non-competitive inhibitor that inactivates RNase by non-covalent binding. Has been used to improve cDNA synthesis and in vitro RNA synthesis, increase yields of polysomes, and aid in the preparation of RNase-free antibodies. Inhibits RNases A, B, and C. Does not inhibit RNase T1 and S1 nuclease from Aspergillus.

Other Notes

One unit is defined as the amount of material that will inhibit 50% of the activity of 5 ng of RNase A.
Saxena, S.K., et al. 1992. J. Biol. Chem. 267, 21982.
Lee, F.S., et al. 1989. Biochemistry 28, 225.

Physical form

In 50 mM KCl, 20 mM HEPES, 8 mM DTT, 50% glycerol.

Preparation Note

Maintain at -20°C. Avoid freezing solutions. Addition of DTT is recommended to maintain 8 mM level.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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