MAB16985

Anti-MCAM Antibody, clone P1H12

Name: Anti-MCAM Antibody, clone P1H12
Brand: MM

clone P1H12, Chemicon^®, from mouse

Synonym(s):

CD146 antigen, Cell surface glycoprotein P1H12, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen, melanoma adhesion molecule, melanoma cell adhesion molecule

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Product Name

Anti-MCAM Antibody, clone P1H12, clone P1H12, Chemicon^®, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

P1H12, monoclonal

species reactivity

mouse, canine, human

should not react with

rat

packaging

antibody small pack of 25 μg

manufacturer/tradename

Chemicon^®

technique(s)

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

suitability

not suitable for immunohistochemistry (Paraffin)

NCBI accession no.

NM_006500.2

UniProt accession no.

P43121

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Analysis Note

Control
HUVEC cells.

Routinely evaluated by Western Blot on HUVEC lysates.

Western Blot Analysis:
1:500 dilution of this lot detected MCAM on 10μg of HUVEC lysates.

Application

Anti-MCAM Antibody, clone P1H12 detects level of MCAM & has been published & validated for use in ELISA, FC, IC, IH, IP & WB.

Immunocytochemistry:
1-10 μg/mL of a previous lot worked in immunocytochemistry.Works best on EDTA or Trypsin lifted endothelial cells.

Immunohistochemistry:
1-10 µg/mL. 4% PFA for 30min RT or <2hrs at 4°C. Block w/ 1%BSA/0.2% tween20/PBS for 30min. Works well in frozen tissue; fixed or unfixed.

Immunoprecipitation:
1-10 μg/mL of a previous lot worked in immunoprecipitation.

ELISA:
1-10 μg/mL of a previous lot worked in ELISA.

FACS Analysis:
1-10 μg/mL of a previous lot worked in FACS.

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Detects circulating endothelial cells in human. Expected to cross-react with dog & mouse.

General description

MUC18 antigen (CD146), a member of the immuoglobulin superfamily, is an intrinsic membrane glycoprotein of 110-120 kDa found on the surface of endothelial cells, bone marrow fibroblasts and various melanomas. MUC18 has been used as a marker of tumor progression in human melanoma because expression in those tumors correlates strongly with poor prognosis and the development of metastic disease. In addition, a number of human T, B and myeloid leukemic cell lines seem to express MUC18. The close structural relationship with N-CAM and related molecules suggests that MUC18 may be also a developmentally regulated cell adhesion molecule (Melanoma adhesion molecule or MCAM).

110 kDa

Immunogen

Immunization of mice with HUVEC.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Purified mouse monoclonal IgG1 in buffer containing 0.02 M PB with 0.25 M NaCl, pH=7.6, with 0.1% sodium azide.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Shedding of endothelial protein C receptor contributes to vasculopathy and renal injury in lupus: in vivo and in vitro evidence.

Carlos A Sesin et al.

Kidney international, 68(1), 110-120 (2005-06-16)

Candidate biomarkers for vasculopathy in systemic lupus erythematosus (SLE) include circulating endothelial cells and the recently identified endothelial protein C receptor (EPCR) which, when shed, promotes a thrombotic diathesis. This study sought correlation between plasma levels of soluble EPCR and

Melanoma cell adhesion molecule identifies encephalitogenic T lymphocytes and promotes their recruitment to the central nervous system.

Catherine Larochelle et al.

Brain : a journal of neurology, 135(Pt 10), 2906-2924 (2012-09-15)

In multiple sclerosis, encephalitogenic CD4(+) lymphocytes require adhesion molecules to accumulate into central nervous system inflammatory lesions. Using proteomic techniques, we identified expression of melanoma cell adhesion molecule (MCAM) on a subset of human effector memory CD4(+) lymphocytes and on

Murine endothelial cell lines as models of tumor endothelial cells.

Jennifer Walter-Yohrling et al.

Clinical cancer research : an official journal of the American Association for Cancer Research, 10(6), 2179-2189 (2004-03-26)

Identification of appropriate models for in vivo and in vitro preclinical testing of inhibitors of tumor angiogenesis and progression is vital to the successful development of anticancer therapeutics. Although the focus is on human molecular targets, most preclinical in vivo

Primary and immortalised human pancreatic islet endothelial cells: phenotypic and immunological characterisation.

E Favaro et al.

Diabetologia, 48(12), 2552-2562 (2005-11-18)

Studies on the biology of the microvascular endothelial cells (MECs) that surround and penetrate the pancreatic islets are hampered by difficulties in isolating and culturing large numbers of pure cells. We aimed to morphologically and functionally characterise primary MECs purified

Isolation and characterization of human fetal myoblasts.

Ariya D Lapan et al.

Methods in molecular biology (Clifton, N.J.), 798, 3-19 (2011-12-02)

Dissociated human fetal skeletal muscle contains myogenic cells, as well as non-myogenic cells such as adipocytes, fibroblasts, and lymphocytes. It is therefore important to determine an efficient and reliable isolation method to obtain a purer population of myoblasts. Toward this

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Global Trade Item Number

SKU	GTIN
MAB16985	04053252466083
MAB16985-25UG	04054839337543

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