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93352

Trizma® base

≥99.0% (T), crystalline, aminopeptidase substrate, BioChemika

Synonym(s):

2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

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About This Item

Linear Formula:
NH2C(CH2OH)3
CAS Number:
77-86-1
Molecular Weight:
121.14
NACRES:
NA.25
PubChem Substance ID:
329770229
UNSPSC Code:
12352104
EC Number:
201-064-4
MDL number:
MFCD00004679
Beilstein/REAXYS Number:
741883
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Product Name

Trizma® base, ≥99.0% (T)

description

aminopeptidase substrate

Quality Segment

200

product line

BioChemika

assay

≥99.0% (T)

form

crystalline

loss

≤1% loss on drying, 110 °C

pH

10.5-12.0(4 m in water, 25 °C)

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.), 168-172 °C

solubility

H2O: 1 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤50 mg/kg, sulfate (SO42-): ≤50 mg/kg

cation traces

Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Zn: ≤5 mg/kg

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Application

Trizma® base was used as buffer for the following studies:
  • Electrophoretic transfer for the specific identification of isozymes of starch debranching enzyme, α-amylase and 9-amylase.
  • Electrophoretic separation of lipoproteins in polyacrylamide gels.
  • Preparation of TRIS buffer having pH 8.
It may be used to compose DN buffer for DNA nick-end labeling of tissue sections.

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCP3624
BCCN7869
BCCL4655
BCCL7499
BCCL1560

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