A9434
Ammonium chloride
Molecular Biology, suitable for cell culture, ≥99.5%
Synonym(s):
Salmiac
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About This Item
Linear Formula:
NH4Cl
CAS Number:
Molecular Weight:
53.49
NACRES:
NA.31
PubChem Substance ID:
eCl@ss:
38050207
UNSPSC Code:
12352302
EC Number:
235-186-4
MDL number:
Beilstein/REAXYS Number:
4371014
Assay:
≥99.5%
Grade:
Molecular Biology
Form:
powder or crystals
grade
Molecular Biology
Quality Level
vapor density
1.9 (vs air)
vapor pressure
1 mmHg ( 160.4 °C)
assay
≥99.5%
form
powder or crystals
technique(s)
cell culture | mammalian: suitable
mp
340 °C (subl.) (lit.)
anion traces
phosphate (PO43-): ≤2 ppm, sulfate (SO42-): ≤20 ppm
cation traces
Fe: ≤2 ppm, heavy metals (as Pb): ≤5 ppm
foreign activity
DNase, RNase, protease, none detected
SMILES string
N.Cl
InChI
1S/ClH.H3N/h1H;1H3
InChI key
NLXLAEXVIDQMFP-UHFFFAOYSA-N
Application
Ammonium chloride has been used:
- as an inhibitor of endosome acidification in vero cells
- as an autophagy inhibitor in vascular smooth muscle cell (VSMC)
- in the depletion and lysis of erythrocytes in testis parenchyma homogenate
Biochem/physiol Actions
Ammonium chloride is one of the three principal components of the nitrogen cycle. Ammonium chloride administration promotes creatinine and urea clearance andinduces metabolic acidosis in mice. Excess of ammonium chloride inhibits Kreb′s pathway in brain and depletes ATP in astrocytes. Ammonium chloride inhibits acidification in the endosome-lysosome system. Ammonium chloride is a hemolysing agent used for the lysis of erythrocytes.
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signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2
Storage Class
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Aqueous ammonia and ammonium chloride hydrates: Principal infrared spectra
Max JJ and Chapados C
Journal of Molecular Structure, 1046, 124-135 (2013)
Effect of ammonium chloride and dietary phosphorus in the azotaemic rat. I. Renal function and biochemical changes
Jara A, et al.
Nephrology, Dialysis, and Transplantation, 19(8), 1986-1992 (2004)
Infectious bronchitis virus entry mainly depends on clathrin mediated endocytosis and requires classical endosomal/lysosomal system
Wang H, et al.
Virology, 528, 118-136 (2019)