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Merck

C9205

Anti-Glial Fibrillary Acidic Protein (GFAP)−Cy3™ antibody, Mouse monoclonal

clone G-A-5, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody produced in mouse, Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP)−Cy3™ antibody produced in mouse, Anti-ALXDRD, Anti-GFAP

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203

Product Name

Anti-Glial Fibrillary Acidic Protein (GFAP)−Cy3™ antibody, Mouse monoclonal, clone G-A-5, purified from hybridoma cell culture

biological source

mouse

conjugate

CY3 conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

G-A-5, monoclonal

form

buffered aqueous solution

species reactivity

human, pig, rat

technique(s)

direct immunofluorescence: 1:400 using alcohol-fixed, paraffin-embedded rat brain sections

isotype

IgG1

UniProt accession no.

P14136

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... GFAP(2670)
rat ... Gfap(24387)

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Application

Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) (mouse IgG1 isotype) conjugated to Cy3 has been used in
  • immunohistochemistry
  • immunoblotting
  • immunolabelling,
  • immunocytochemistry
  • immunofluorescence

Biochem/physiol Actions

Glial Fibrillary Acidic Protein (GFAP) is expressed in several glial-related intracranial neoplasms such as astrocytomas and to a variable extent in ependymomas, medulloblastomas, choroid plexus papillomas, gangliogliomas, mixed gliomas, hemangioblastomas, pineocytomas, pineoblastomas, pituitary adenomas, retinoblastomas, oligodendrogliomas and in a small minority of peripheral nerve sheath tumors (schwannomas and neurofibromas). It may also be found in pleomorphic adenomas and ovarian and testicular teratomas.
The antibody reacts specifically with GFAP in immunoblotting assays and labels astrocytes, Bergmann glia cells and chondrocytes of elastic cartilage in immunohistochemical staining. The antibody reacts with glial specific antigen in frozen or alcohol-fixed tissue sections.
The antibody reacts specifically with GFAP in immunoblotting assays and labels astrocytes, and chondrocytes of elastic cartilage in immunohistochemical staining. The antibody reacts with glial specific antigen in frozen or alcohol-fixed tissue sections.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) (mouse IgG1 isotype) conjugated to Cy3 is a purified mouse monoclonal antibody conjugated to Cy3 reactive dye. It is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. GFAP, in the central nervous system, is found in developing, normal, reactive and neoplastic astrocytes and ependymal cells. It is expressed in developing oligodendrocytes in the peripheral nervous system. GFAP is found in some Schwann cells, enteric glial cells, Müller glia of the retina, and the satellite cells of human sensory ganglia. It has been demonstrated in some non-glial cells as well.

Immunogen

GFAP from pig spinal cord

Legal Information

Cy is distributed under license from Amersham Biosciences Limited.
Cy3 is a trademark of Cytiva

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000415055
0000415054
0000415055
0000415054
0000389732

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Sulfonylurea Receptor 1, Transient Receptor Potential Cation Channel Subfamily M Member 4, and KIR6. 2: Role in Hemorrhagic Progression of Contusion
Gerzanich V, et al.
Journal of Neurotrauma, 36(7), 1060-1079 (2019)
J Michael Gee et al.
Frontiers in molecular neuroscience, 8, 10-10 (2015-05-01)
Complex interactions between networks of astrocytes and neurons are beginning to be appreciated, but remain poorly understood. Transgenic mice expressing fluorescent protein reporters of cellular activity, such as the GCaMP family of genetically encoded calcium indicators (GECIs), have been used
Expression of vimentin and glial fibrillary acidic protein in central nervous system development of rats
Luo H, et al.
Asian Pacific Journal of Tropical Medicine, 10(12), 1185-1189 (2017)
Aixa F Rivera-Pagán et al.
PloS one, 10(4), e0125195-e0125195 (2015-04-18)
Excitotoxicity due to glutamate receptor over-activation is one of the key mediators of neuronal death after an ischemic insult. Therefore, a major function of astrocytes is to maintain low extracellular levels of glutamate. The ability of astrocytic glutamate transporters to
Sequel of spontaneous seizures after kainic acid-induced status epilepticus and associated neuropathological changes in the subiculum and entorhinal cortex
Drexel M, et al.
Neuropharmacology, 63(5), 806-817 (2012)
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