D5905

3,3′-Diaminobenzidine tetrahydrochloride

Name: 3,3′-Diaminobenzidine tetrahydrochloride
Brand: SIGMA

peroxidase substrate, chromogenic, tablet

Synonym(s):

3,3′,4,4′-Biphenyltetramine tetrahydrochloride, 3,3′,4,4′-Tetraaminobiphenyl tetrahydrochloride, DAB

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All Photos(1)

About This Item

Linear Formula:

(NH₂)₂C₆H₃C₆H₃(NH₂)₂ · 4HCl

Molecular Weight:

360.11

NACRES:

NA.83

PubChem Substance ID:

24893981

UNSPSC Code:

12352204

EC Number:

231-018-9

MDL number:

MFCD00012969

Beilstein/REAXYS Number:

1212988

Product Name

3,3′-Diaminobenzidine tetrahydrochloride, tablet, 10 mg substrate per tablet

InChI

1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H

InChI key

KJDSORYAHBAGPP-UHFFFAOYSA-N

SMILES string

Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

form

tablet

solubility

water: 1 tablet/15 mL (may contain small insoluble particles)

storage temp.

−20°C

Quality Level

200

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Related Categories

Proteins & Enzymes

Detection Substrates & Enzymes

Application

Reagent for spectrophotometric determination of selenium.

3,3′-Diaminobenzidine tetrahydrochloride has been used for immunohistochemistry.

Biochem/physiol Actions

3,3′-Diaminobenzidine tetrahydrochloride is a chromogenic substrate, which is commonly used in immunoperoxidase tests.

Peroxidase substrate suitable for use in immunoblotting and immunohistological staining procedures. This substrate produces an insoluble end product that is brown in color and can be observed visually.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Preparation Note

Dissolve 1 tablet in 15 ml of Tris-buffered saline, pH 7.6. Add 12 μl of fresh 30% hydrogen peroxide. DAB and hydrogen peroxide concentration may be adjusted to suit individual applications.

pictograms

GHS08,GHS07

signalword

Danger

hcodes

H302,H319,H341,H350

pcodes

P202 - P264 - P280 - P301 + P312 - P305 + P351 + P338 - P308 + P313

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

ppe

Eyeshields, Gloves, type N95 (US)

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