E5160
Epidermal Growth Factor from mouse
≥90% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell cutlure
Synonym(s):
Mouse EGF, Mouse Epidermal Growth Factor
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.77
Biological source:
mouse
Recombinant:
expressed in E. coli
Assay:
≥90% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
~6 kDa
Impurities:
≤1 EU/μg endotoxin (Protein)
Product Name
Epidermal Growth Factor from mouse, EGF
biological source
mouse
Quality Segment
recombinant
expressed in E. coli
assay
≥90% (SDS-PAGE)
form
lyophilized powder
potency
0.05-1 ng/mL ED50/EC50
mol wt
~6 kDa
packaging
pkg of 100 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/μg endotoxin (Protein)
color
white
solubility
water: soluble 0.10 mL, clear, colorless
UniProt accession no.
storage temp.
−20°C
Gene Information
mouse ... Egf(13645)
General description
Epidermal Growth Factor (EGF) is a small (~6 kDa) recombinant mitogenic polypeptide expressed in E. coli and supplied as a lyophilized powder. EGF is widely distributed in mammalian tissues and mediates cellular responses through ErbB family receptor tyrosine kinases, including EGFR/ErbB1. This product is suitable for in vitro mammalian cell culture research applications.
Application
Recombinant mouse EGF has been used as a supplement in cell culture research applications, including basal N2B27 medium for culturing differentiated mouse embryonic stem (E14) cells.
Biochem/physiol Actions
Epidermal Growth Factor (EGF) is a mitogenic factor involved in the regulation of cellular proliferation, differentiation, and survival in vitro. EGF exhibits activity across multiple mammalian cell types, including fibroblasts, epithelial cells, and endothelial cells, through signaling via EGF receptor family pathways.
Physical form
The product is lyophilized from a 0.2 μm-filtered solution of phosphate buffered saline (PBS), pH 7.4.
Storage Class
11 - Combustible Solids
wgk
WGK 1
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