E8655
Anti-E6AP antibody, Mouse monoclonal
clone E6AP-330, purified from hybridoma cell culture
Synonym(s):
Anti-E2F-6
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About This Item
Conjugate:
unconjugated
Clone:
E6AP-330, monoclonal
Application:
ARR, ELISA (i), ICC, IP, WB
Citations:
44
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
E6AP-330, monoclonal
form
buffered aqueous solution
mol wt
antigen ~100 kDa
species reactivity
human, mouse, rat, monkey
technique(s)
immunocytochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 1-2 μg/mL using total cell extract from 293T cells
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... E2F6(1876)
mouse ... E2f6(50496)
rat ... E2f6(313978)
General description
E6AP is an E3 ubiquitin ligase that is expressed by the UB3A gene. Inhibiton or alterations of the UB3A gene may cause a neurological disorder called the Angelman Syndrome. E6AP interacts with E1 and E2 enzymes to mediate ubiquitination of proteins marked for degradation. E6AP also binds with the E6 viral protein present in HPV-infected cells.
Monoclonal Anti-E6AP antibody is a useful tool for the study of E6AP and its function in protein degradation. This antibody is specific for E6AP protein in rat, mouse, human and monkey.
Monoclonal Anti-E6AP antibody is a useful tool for the study of E6AP and its function in protein degradation. This antibody is specific for E6AP protein in rat, mouse, human and monkey.
Immunogen
human full-length recombinant E6AP.
Application
Monoclonal Anti-E6AP antibody is suitable for use in western blot (1-2 μg/mL using total cell extract from 293T cells), immunocytochemistry, immunoblotting, immunoprecipitation, indirect ELISA and microarray.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
The role of TP53 in Cervical carcinogenesis.
Tommasino, M., et al.
Human Mutation, 21, 307-312 (2003)
Yi Sun
Cancer biology & therapy, 2(6), 623-629 (2003-12-23)
E3 ubiquitin ligases are a large family of proteins that can be classified into three major structurally distinct types: N-end rule E3s, E3s containing the HECT (Homology to E6AP C-Terminus) domain, and E3s with the RING (Really Interesting New Gene)
B Dan et al.
Neuropediatrics, 34(4), 169-176 (2003-09-16)
Angelman syndrome is characterised by neurodevelopmental impairment (with or without epileptic seizures) associated with functional deficit of the UBE3A gene. Different mechanisms of UBE3A inactivation correlate with clinical phenotypes of varying severity. However, three distinctive, highly consistent electroencephalographic rhythmic patterns