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Merck

F1804

ANTI-FLAG® M2 antibody, Mouse monoclonal

1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

Synonym(s):

flag antibody, Anti-ddddk, Anti-dykddddk, Monoclonal ANTI-FLAG® M2

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352203

Product Name

ANTI-FLAG® M2 antibody, Mouse monoclonal, 1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

biological source

mouse

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

purified by

affinity chromatography (affinity tag purification)

species reactivity

all

concentration

1 mg/mL

isotype

IgG1

immunogen sequence

DYKDDDDK

shipped in

wet ice

storage temp.

−20°C

Quality Level

200

Related Categories

Application

For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting, immunoprecipitation (IP) , immunohistochemisty, immunofluorescence and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.

Western Blotting and EIA

Learn more product details in our FLAG® application portal.

Biochem/physiol Actions

Binding site:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C

General description

The ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus, and internal sites of the fusion protein.

Method of purification - Affinity tag purification

Immunogen

FLAG; peptide sequence DYKDDDDK

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Physical form

Contains 50% glycerol in phosphate buffered saline.

Preparation Note

Immediately before use, dilute in Tris buffered saline (TBS), pH 8.0, with 3% nonfat Milk

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000502386
0000502386
0000502242
0000502242
0000472993

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