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G6751

α-Glycerophosphate Dehydrogenase from rabbit muscle

Name: α-Glycerophosphate Dehydrogenase from rabbit muscle
Brand: SIGMA

Type I, ammonium sulfate suspension, 100-300 units/mg protein

Synonym(s):

sn-Glycerol-3-phosphate Dehydrogenase from rabbit muscle, sn-Glycerol-3-phosphate:NAD⁺ 2-oxidoreductase

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About This Item

CAS Number:

9075-65-4

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-982-3

MDL number:

MFCD00040474

EC Number:

1.1.1.8 (BRENDA, IUBMB)

Specific activity:

100-300 units/mg protein

Biological source:

rabbit muscle

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Properties

biological source

rabbit muscle

Quality Segment

200

type

Type I

form

ammonium sulfate suspension

specific activity

100-300 units/mg protein

storage condition

(Tightly closed)

technique(s)

activity assay: suitable

color

white

foreign activity

Lactic dehydrogenase, pyruvate kinase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%, Triosephosphate isomerase ≤0.02%

storage temp.

2-8°C

Description

General description

α-Glycerophosphate dehydrogenase is a membrane protein and a flavin-linked primary dehydrogenase.

Research area: Cell Signaling

Application

α-Glycerophosphate Dehydrogenase from rabbit muscle has been used:

in the reaction mixture to measure the glycerol kinase activity
to demonstrate compartmentalized enzymatic reactions where NADH is involved
in the reaction mixture assay for L-fuculose-1-phosphate aldolase

Biochem/physiol Actions

α-Glycerophosphate dehydrogenase catalyzes the oxidation of L-α-glycerophosphate (Glp) to form dihydroxyacetone phosphate (DHAP) and hydrogen peroxide (H2O2). This enzyme is associated with respiratory electron transport chain, glycolysis, and phospholipid metabolism.

Physical form

Crystalline suspension in 3.2 M (NH₄)₂SO₄ and 0.1 g/L EDTA solution, pH 6.0

Preparation Note

Prepared by modification of the method of Fondy.

Analysis Note

Protein determined by biuret.

Other Notes

One unit will convert 1.0 μmole of dihydroxyacetone phosphate to α-glycerophosphate per min at pH 7.4 at 25 °C.

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Pictograms

GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)