P4170

Propidium iodide

Name: Propidium iodide
Brand: SIGMA

≥94.0% (HPLC), fluorescent dye, powder

Synonym(s):

3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide

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About This Item

Empirical Formula (Hill Notation):

C₂₇H₃₄I₂N₄

CAS Number:

25535-16-4

Molecular Weight:

668.39

NACRES:

NA.77

PubChem Substance ID:

57654524

UNSPSC Code:

41106305

EC Number:

247-081-0

MDL number:

MFCD00011921

Beilstein/REAXYS Number:

3843838

Product Name

Propidium iodide, ≥94.0% (HPLC)

InChI key

XJMOSONTPMZWPB-UHFFFAOYSA-M

InChI

1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1

SMILES string

[I-].[I-].CC[N+](C)(CC)CCC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14

assay

≥94.0% (HPLC)

form

powder

mp

220-225 °C (dec.) (lit.)

storage temp.

2-8°C

Quality Level

200

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Related Categories

Bioactive Small Molecules

Application

Propidiumiodide has been used: to detect cell cycle by flow cytometry and γH2AXand pH3 analysis,apoptosis assay in hepatic stellate cells (HSCs), in stainingbuffer/solution to stain the cells for cell cycle analysis

Features and Benefits

This compound is a featured product for Apoptosis research. Click here to discover more featured Apoptosis products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

General description

Propidium iodide (PI) is a fluorescent dye thatcan be used for staining nucleic acids. It is impermeable in viable orintact cells. It can penetrate easily into dead, dying, or membrane-compromisedcells. PI has the capacity to bind easily to DNA.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

pictograms

GHS08

signalword

Warning

hcodes

H341

pcodes

P201 - P202 - P280 - P308 + P313 - P405 - P501

Hazard Classifications

Muta. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Tunicamycin Induces Hepatic Stellate Cell Apoptosis Through Calpain-2/Ca2 +-Dependent Endoplasmic Reticulum Stress Pathway.

Haiying Liu et al.

Frontiers in cell and developmental biology, 9, 684857-684857 (2021-10-05)

It has been reported that calpain/caspase-mediated apoptosis induced by endoplasmic reticulum stress (ERS) in hepatic stellate cells (HSCs) by previous studies. At present, the activation of HSC is an important cause of liver fibrosis, and the induction of HSC apoptosis

An Annexin V-FITC-Propidium Iodide-Based Method for Detecting Apoptosis in a Non-Small Cell Lung Cancer Cell Line.

Robin Kumar et al.

Methods in molecular biology (Clifton, N.J.), 2279, 213-223 (2021-03-09)

Annexin V and propidium iodide staining is widely used for determining the cellular death through apoptosis. In the presence of Ca2+ ions, annexin V has a strong binding affinity for phosphatidylserine, a membrane phospholipid that during apoptosis is translocated from

Ectopic activation of WNT signaling in human embryonal carcinoma cells and its effects in short- and long-term in vitro culture.

Yaser Atlasi et al.

Scientific reports, 9(1), 11928-11928 (2019-08-17)

Human embryonal carcinoma (EC) cells comprise the pluripotent stem cells of malignant non-seminomatous germ cell tumors (GCTs) and represent the malignant counterpart of embryonic stem cells (ESCs). WNT/β-catenin signaling has been implicated in regulating adult and embryonic stem cells although

Illuminating touch deposits through cellular characterization of hand rinses and body fluids with nucleic acid fluorescence.

Julia Burrill et al.

Forensic science international. Genetics, 46, 102269-102269 (2020-03-11)

Forensic DNA typing from touched or handled items in routine casework is increasing as the sensitivity of detection techniques improves. Our understanding of the cellular/acellular content of touch deposits and the origins of the DNA therein is still limited. This

Ablation of core fucosylation attenuates the signal transduction via T cell receptor to suppress the T cell development.

Wei Liang et al.

Molecular immunology, 112, 312-321 (2019-06-24)

Precise glycosylation plays a crucial and distinctive role in thymic T cell development. The core fucosylation is dramatically up-regulated at the transition from CD4-CD8- (DN) to CD4+CD8+ (DP) in the thymic development. Ablation of core fucosylation in T cells did

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