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SRP0360

PLK1 Polo Box Domain active human

Name: PLK1 Polo Box Domain active human
Brand: SIGMA

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Synonym(s):

STPK13, polo-like kinase 1

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About This Item

NACRES:

NA.32

UNSPSC Code:

12352200

Assay:

≥90% (SDS-PAGE)

Biological source:

human

Recombinant:

expressed in E. coli

Concentration:

2.9 mg/mL

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Properties

biological source

human

recombinant

expressed in E. coli

assay

≥90% (SDS-PAGE)

form

aqueous solution

mol wt

53 kDa

packaging

pkg of 100 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

2.9 mg/mL

NCBI accession no.

NM_005030

UniProt accession no.

P53350

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PLK1(5347)

Description

General description

Polo-like kinase 1 (PLK1) belongs to the PLK family. It comprises polo-box domain (PBD). PBD, in turn, contains polo boxes (PB1 and PB2) with a 6-stranded β sandwich and an α helix as structural elements. The PLK1 gene is mapped to human chromosome 16p12.2.

Human Plk1 polo box domain (GenBank Accession No. NM_005030), amino acids 367-end with N-terminal GST-tag, MW=53 kDa, expressed in an E. coli expression system.

Application

PLK1 Polo Box Domain active human has been used in the in vitro binding assay with various synthetic peptides coupled to streptavidin beads. It has also been used in binding studies with fluorescein-labeled substrate peptide 5-carboxyfluorescein-GPMQSpTPLNG to test the effect of various inhibitors.

Biochem/physiol Actions

Polo-like kinase 1 (PLK1) is a mitotic kinase that mediates the regulation of various events in mitosis including chromosome segregation, cytokinesis, centrosome maturation, and separation. It activates cell division cycle 25 (CDC25) phosphatase which further regulates mitotic entry. Elevated expression of PLK1 is observed in many tumors.

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c