Anti-Farnesyl Antibody

Protein isoprenylation is a post-translational modification that affects about 0.5% of cellular proteins and is essential for the biological activity of proteins. Two enzymes catalyze theattachment of two prenyl groups to the sulfhydryl group of carboxyl-terminal cysteine groups. Proteins which are prenylated by these enzymes have a distinct motif at the C-terminal of the protein, C-A1-A2-X (C = Cysteine, A 1 & A2 = aliphatic amino acids). The two enzymes involved in this transfer are farnesyltransferase and geranylgeranyltransferase. These transfer a 15 carbon farnesyl or a 20 carbon geranygeranyl, respectively, from aprenyl-pyrophosphate to the protein. Examples of proteins containing this C-A-A-X motif are members of the Ras small G protein family, the nuclear lamins and the gamma subunit oftrimeric G proteins. Prenylation of proteins is necessary for membrane association of proteins as well as protein-protein interactions and the nature of the linked isoprenyl group can influence the protein interactions, such as the interaction between G proteins and receptors.

Antibody developed using farnesyl cysteine conjugated to KLH.

Anti-Farnesyl Antibody detects level of Farnesyl & has been published & validated for use in ELISA & IF.

ELISA

Immunofluoresecence

Optimal working dilutions must be determined by end user.

Research Category
Signaling

Research Sub Category
MAP Kinases

Recognizes farnesyl motif derived from isoprenylated C-A-A-X sequence. Also cross-reacts with geranylgeranyl motif. Detects KLH as well.

Format: Purified

Purified immunoglobulin. Sterile liquid in phosphate buffered saline and 0.08% sodium azide.

Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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