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Merck

SNAP2MINI

SNAP id® 2.0 Protein Detection System-Mini

7.5 x 8.4 cm, Unique vacuum-driven technology & a built-in flow distributor actively drive reagents through the membrane

Synonym(s):

Protein detection kit

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About This Item

UNSPSC Code:
41116010
eCl@ss:
42029053
NACRES:
NB.22
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Product Name

SNAP id® 2.0 Protein Detection System-Mini (7.5 x 8.4 cm), Developed to meet the needs of our Western blotting customers, the SNAP i.d. 2.0 system produces blots of a very high quality. Unique vacuum-driven technology & a built-in flow distributor actively drive reagents through the membrane..

manufacturer/tradename

SNAP id®

technique(s)

western blot: suitable

compatibility

for use with Commercially available blocking reagents, for use with Luminata Western HRP Substrates, for use with Nitrocellulose, for use with PVDF (Immobilon membranes), for use with blØk<TMSYMBOL></TMSYMBOL>-CH Buffer (cat. no. WBAVDCH01), for use with blØk<TMSYMBOL></TMSYMBOL>-FL Buffer (cat. no. WBAVDFL01), for use with blØk<TMSYMBOL></TMSYMBOL>-PO Buffer (cat. no. WBAVDP001), for use with commercially available detection reagents

detection method

chemiluminescent, colorimetric, fluorometric

shipped in

ambient

storage temp.

room temp

General description

The newly developed SNAP id 2.0 Protein Detection System is the second generation of the SNAP i.d. method for detecting immunoreactive proteins on western blots. With this unique vacuum-driven system, the length of time required for immunodetection is greatly reduced. The classical western blotting time frame of 4- 24 hours is now accomplished in 30 minutes with no quality and signal compromisation. All immunodetection steps after protein transfer to a membrane (i.e., blocking, washing, and primary and secondary antibody incubations) can be performed with the SNAP id 2.0 system. SNAP id 2.0 Mini Blot Holder accepts up to 7.5 × 8.4 cm blots.

Application

SNAP id 2.0 Protein Detection System-Mini (7.5 x 8.4 cm) has been used in western blot analysis.

Features and Benefits

SNAP id 2.0 Protein Detection System Features:
  • Processing of up to four blots at a time on a vacuum base with two individually controlled sides
  • Comprises of three removable blot holding frame sizes: MultiBlot, Mini, and Midi, to accommodate different blot sizes
  • Disposable blot holders, sized for MultiBlot, Mini, and Midi frames
  • Blot spacer required with first-generation SNAP id system is now integrated into the new blot holder
  • Extended and off-line blot processing options: frames have lids and can be removed from the base for extended incubation (one hour to overnight), incubation in a shaker, or incubation at 4 °C
  • Stackable frames so multiple blots can be processed at the same time;30-minute immunodetection with uniform signal across the blot
  • Greater than 80% antibody recovery using the SNAP id 2.0 Antibody Collection Trays
  • Compatible with nitrocellulose and polyvinylidene fluoride (PVDF) membranes
  • Works with the most blocking buffers and visualization methodologies (e.g. chemiluminescence, fluorescence, or colorimetric)

Packaging

This system includes the SNAP id® 2.0 Base, two Mini Blot Holding Frames, two Antibody Collection Trays, vacuum tubing, Rolling Pad & Blot Roller, two Wetting Trays and a Quick Start Guide

Other Notes

Replaces: WBAVDBASE
The SNAP id® 2.0 Protein Detection System is intended for research use only. It is not for use in diagnostic procedures.

Legal Information

SNAP id is a registered trademark of Merck KGaA, Darmstadt, Germany




Certificates of Analysis (COA)

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Related Content

Antibody reuse for Western blotting is a common practice for many researchers. While many antibodies lose potency with time or degrade even faster due to improper storage conditions, it is important to recognize the potential value of recovering the primary antibody for possible reuse in some experiments. The SNAP i.d.® 2.0 system is not only able to reduce the immunodetection processing time, but its flexibility lets you combine conditions used in the standard immunodetection protocol and also allows the collection of antibody for future reuse. Here, we compare the antibody recovery and reuse in the standard immunodetection protocol with the antibody recovery and reuse in SNAP i.d.® system using the extended protocol and the original SNAP i.d.® protocol.

There’s so much room for experimental variability in traditional immunodetection workflows. For your peace of mind – and ours – we designed the SNAP i.d.® 2.0 system to streamline your Western blot and immunohistochemistry experiments. The concept is simple: a vacuum-driven flow of blocking, antibody, and washing solutions reduces slide and membrane handling. That means a lot less shaking, dipping, pouring and waiting. And now you can process multiple blots and slides in parallel, so it’s easy to apply consistent conditions across experiments.

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Global Trade Item Number

SKUGTIN
SNAP2MINI04053252011177