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Merck

72091

NBT-BCIP® solution

BioReagent

Synonym(s):

BCIP®/NBT, NBT-X-phosphate

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About This Item

UNSPSC Code:
12171500
PubChem Substance ID:
329763805
NACRES:
NA.21

Key Documents

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SMILES string

[Cl-].[Cl-].Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23.COc4cc(ccc4-[n+]5nc(nn5-c6ccc(cc6)[N+]([O-])=O)-c7ccccc7)-c8ccc(c(OC)c8)-[n+]9nc(nn9-c%10ccc(cc%10)[N+]([O-])=O)-c%11ccccc%11

InChI

1S/C40H30N10O6.C8H6BrClNO4P.C7H9N.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6;;/h3-26H,1-2H3;1-3,11H,(H2,12,13,14);2-5H,8H2,1H3;2*1H/q+2;;;;/p-2

InChI key

GDPIIVBVMSORRQ-UHFFFAOYSA-L

product line

BioReagent

form

liquid

technique(s)

microbe id | staining: suitable

solubility

0.1 M Tris-HCl, pH 7.4: 0.0175 mL/mL, clear

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

Quality Level

100

Related Categories

Physical form

solution of 18.8 mg/ml nitro-blue tetrazolium chloride; 9.4 mg/ml 5-bromo-4-chloro-3-indolylphosphate toluidine salt in 67% DMSO

Other Notes

Chromogenic phosphatase substrate producing a blue-colored precipitate at the site of enzymatic activity

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

152.6 °F - closed cup

flash_point_c

67 °C - closed cup

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
BCCB8209
BCBV6055
BCBS2526V
BCBP6799V
BCBN5576V

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Ying Wu et al.
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The class V chitin synthase is unique because it has a myosin motor-like domain fused to its catalytic domain. The biochemical properties of this enzyme and its function remain undefined beyond the knowledge that it is the only single chitin
Hyun-Jung Kim et al.
The International journal of developmental biology, 53(7), 993-1002 (2009-07-15)
The current-producing cells of the electric organ, i.e., electrocytes, in Sternopygus macrurus derive from skeletal muscle fibers. Mature electrocytes are not contractile, but they do retain some muscle proteins, are multinucleated, and receive cholinergic innervation. Electrocytes express the myogenic regulatory
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D Thompson et al.
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A general method is described for the electrophoretic transfer of proteins from stained gels to membranes and subsequent Western detection of specific proteins on the stained membranes. Proteins are separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis, and the gels
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