A0185
Monoclonal Anti-Biotin−Peroxidase antibody produced in mouse
clone BN-34, purified immunoglobulin
Synonym(s):
Monoclonal Anti-Biotin
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About This Item
NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
peroxidase conjugate
Clone:
BN-34, monoclonal
Application:
dot blot
flow cytometry
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
indirect ELISA
western blot (chemiluminescent)
flow cytometry
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
indirect ELISA
western blot (chemiluminescent)
Technique(s):
dot blot: suitable
flow cytometry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300
indirect ELISA: 1:30,000
western blot (chemiluminescent): 1:80,000
flow cytometry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300
indirect ELISA: 1:30,000
western blot (chemiluminescent): 1:80,000
Citations:
21
Product Name
Monoclonal Anti-Biotin−Peroxidase antibody produced in mouse, clone BN-34, purified immunoglobulin
Quality Level
biological source
mouse
conjugate
peroxidase conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
BN-34, monoclonal
packaging
vial of 0.5 mL
technique(s)
dot blot: suitable
flow cytometry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300
indirect ELISA: 1:30,000
western blot (chemiluminescent): 1:80,000
isotype
IgG1
storage temp.
2-8°C
target post-translational modification
unmodified
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Application
May be substituted for avidin to decrease non-specific binding.
Monoclonal Anti-Biotin Peroxidase antibody produced in mouse has been used:
- in immunohistochemistry
- in western blot
- as secondary antibody to detect the presence of aptamer on the membrane
The antibody may be used in amplification techniques, ELISA, immunoblotting, in situ nucleic acid hybridization, flow cytometry, image analysis or confocal microscopy.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.
Biochem/physiol Actions
Biotin is an essential vitamin required for cells in living organisms and in cultures. The high binding affinity of biotin to egg white avidin or bacteria-derived streptavidin has been used to design immunoassays for detecting antigen-antibody interactions. The use of monoclonal anti-biotin IgG can enhance the sensitivity of such avidin-biotin immunoassays. The monoclonal antibody functions by selectively enlarging and bridging the avidin-biotin-enzyme complexes, thereby increasing the signals from substrate conversion. Furthermore, this antibody can be used in many other applications where biotin may be introduced as target label.
Monoclonal Anti-Biotin-Peroxidase antibody specific for biotin conjugated to various proteins. The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immunohistochemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. The product specificity has been evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates.
Monoclonal Anti-Biotin-Peroxidase antibody specific for biotin conjugated to various proteins. The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immunohistochemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. The product specificity has been evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-Biotin (mouse IgG1 isotype) has been derived from the BN-34 hybridoma produced by the used successfully for detection of micro-injected, fusion of mouse myeloma cells and splenocytes from biotin-haptenized cytoskeletal proteins which enable BALB/c mice immunized with biotinylated keyhole limpet hemocyanin (KLH).
The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immunohistochemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. Specificity is evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates.
Immunogen
Biotin-KLH conjugate
Physical form
Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT
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Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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