Anti-14-3-3σ Antibody, clone CS112-2A8

30 kDa

GST fusion protein corresponding to full-length human 14-3-3 sigma

Detect 14-3-3σ using this Anti-14-3-3σ Antibody, clone CS112-2A8 validated for use in IP & WB.

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

Recognizes 14-3-3σ, does not cross-react with other 14-3-3 isoforms.

Format: Purified

Protein G Purified

Protein G Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Control
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

routinely evaluated by immunoblot on RIPA lysates of EGF-stimulated A431 cells

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