444105
Anti-Mannose 6-Phosphate Receptor Mouse mAb (2G11)
liquid, clone 2G11, Calbiochem®
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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
2G11, monoclonal
Species reactivity:
bovine, monkey, human
Application:
—
Citations:
1
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
2G11, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
bovine, monkey, human
should not react with
hamster
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(Immunoblotting (1 µg/mL, non-reducing)
Immunofluorescence
Immunohistochemistry (2-5 µg/mL)
Immunoprecipitation )
isotype
IgG2a
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... M6PR(4074)
General description
Purified mouse monoclonal antibody. Recognizes the mannose 6-phosphate receptor protein.
Recognizes the ~300 kDa mannose 6-phosphate receptor protein.
This Anti-Mannose 6-Phosphate Receptor Mouse mAb (2G11) is validated for use in Immunoblotting, IF, IHC, IP for the detection of Mannose 6-Phosphate Receptor.
Immunogen
Bovine
Epitope: within the extracelluar domain
native bovine cation independent (CI)-mannose 6-phosphate receptor
Application
Immunoblotting (1 µg/ml, non-reducing, see comments)
Immunofluorescence (see comments)
Immunohistochemistry (2-5 µg/ml)
Immunoprecipitation (see comments)
Immunofluorescence (see comments)
Immunohistochemistry (2-5 µg/ml)
Immunoprecipitation (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Dintzis, S., et al. 1994. J. Biol. Chem.269, 12159.
Dintzis, S., and Pfeffer, S. 1990. EMBO J.9, 77.
Dintzis, S., and Pfeffer, S. 1990. EMBO J.9, 77.
Does not detect MPR in hamster (CHO) cells. Not suitable for immunoblotting using reducing conditions. This antibody has also been reported to work for immunoprecipitation and immunofluorescence. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Jennifer Hirst et al.
Human molecular genetics, 24(17), 4984-4996 (2015-06-19)
Adaptor proteins (AP 1-5) are heterotetrameric complexes that facilitate specialized cargo sorting in vesicular-mediated trafficking. Mutations in AP5Z1, encoding a subunit of the AP-5 complex, have been reported to cause hereditary spastic paraplegia (HSP), although their impact at the cellular