Skip to Content
Merck

Key Documents

View All Documentation

8.51025

5-Carboxyfluorescein

Novabiochem®

Synonym(s):

5-Carboxyfluorescein, 5-FAM

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

Empirical Formula (Hill Notation):
C21H12O7
CAS Number:
76823-03-5
Molecular Weight:
376.32
UNSPSC Code:
12352005
NACRES:
NA.22
MDL number:
MFCD00036874
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Quality Level

200

product line

Novabiochem®

assay

≥98% (TLC), ≥99.0% (HPLC)

form

powder

reaction suitability

reagent type: fluorescent-labelling reagent

manufacturer/tradename

Novabiochem®

application(s)

peptide synthesis

storage temp.

2-8°C

SMILES string

O1C3(c4c(cc(cc4)O)Oc5c3ccc(c5)O)c2c(cc(cc2)C(=O)O)C1=O

InChI

1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)

InChI key

NJYVEMPWNAYQQN-UHFFFAOYSA-N

General description

This high purity fluorescent dye (λex494 nm, λem 518 nm) is supplied as a single isomer, which ensures production of labeled products of defined chemical structure, as well as greatly simplifying purification and characterization. FAM should be introduced as the last step in the synthesis to avoid formation of side-products, or be protected by tritylation prior to acylation or hydrazine treatment [1].

Associated Protocols and Technical Articles
Chromogenic Labeling of Synthetic Peptides

Literature references

[1] R. Fischer, et al. (2003) Bioconjugate Chem., 14, 653.

Application

5-Carboxyfluorescein Novabiochem®. CAS 76823-03-5, molar mass 376.32 g/mol.

Analysis Note

Colour (visual): yellow to orange
Appearance of substance (visual): powder
Identity (IR): passes test
Purity (TLC (EtOAc/MeOH/H2O/DIPEA 60 : 30 : 10 : 3)): ≥ 98 %
Assay (HPLC, area%): ≥ 99.0 % (a/a)

To see the solvent systems used for TLC of Novabiochem® products please click here.

Other Notes

Replaces: 01-63-0112

Legal Information

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Still not finding the right product?

Explore all of our products under 5-Carboxyfluorescein

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library