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Merck

AP182R

Donkey Anti-Rabbit IgG Antibody, Rhodamine conjugate, Species Adsorbed

Chemicon®, from donkey

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
eCl@ss:
32160702
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Product Name

Donkey Anti-Rabbit IgG Antibody, Rhodamine conjugate, Species Adsorbed, Chemicon®, from donkey

biological source

donkey

conjugate

TRITC conjugate
rhodamine conjugate

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

rabbit

manufacturer/tradename

Chemicon®

technique(s)

immunofluorescence: suitable

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

Related Categories

Application

Donkey anti-Rabbit IgG Antibody, Rhodamine conjugate, Species Adsorbed is an antibody against Donkey Rabbit IgG for use in IF.
Research Category
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
Suggested dilution for most applications: 1:50-1:200

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Rabbit IgG

WAVELENGTH:
Absorption peak = 550nm, Emission peak = 570 nm.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Physical form

Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, and 0.05% sodium azide.

RECONSTITUTION:
Reconstitute with 500 μL of sterile distilled water.

RHODAMINE/PROTEIN:
Approximately 0.52 (A550/A280)

Preparation Note

Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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hcodes

Hazard Classifications

Aquatic Chronic 3

Storage Class

11 - Combustible Solids

wgk

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A mutation in the nuclear pore complex gene Tmem48 causes gametogenesis defects in skeletal fusions with sterility (sks) mice.
Akiyama, K; Noguchi, J; Hirose, M; Kajita, S; Katayama, K; Khalaj, M; Tsuji, T; Fairfield et al.
The Journal of Biological Chemistry null
Anukul T Shenoy et al.
PLoS pathogens, 13(8), e1006582-e1006582 (2017-08-26)
For over 130 years, invasive pneumococcal disease has been associated with the presence of extracellular planktonic pneumococci, i.e. diplococci or short chains in affected tissues. Herein, we show that Streptococcus pneumoniae that invade the myocardium instead replicate within cellular vesicles
Takeshi Onishi et al.
The Journal of physiology, 597(13), 3441-3455 (2019-05-16)
Neuropathic pain spreads spatially beyond the injured sites, and the mechanism underlying the spread has been attributed to inflammation occurring in the spinal cord. However, the spatial spread of spinal/cortical potentiation induced by conduction block of the peripheral nerves can
N Sae-Ung et al.
European journal of haematology, 74(4), 315-323 (2005-03-22)
Erythropoietin (EPO) and interferon-gamma (IFN-gamma) added to human erythroid progenitor cells purified from peripheral blood (erythroid colony-forming cells; ECFC) significantly reduces apoptosis as assessed by flow cytometry (FCM) using annexin V. To clarify the role of NF-kappaB in the regulation
Petr Dubový et al.
International journal of molecular sciences, 22(14) (2021-07-25)
One of the changes brought about by Wallerian degeneration distal to nerve injury is disintegration of axonal mitochondria and consequent leakage of mitochondrial DNA (mtDNA)-the natural ligand for the toll-like receptor 9 (TLR9). RT-PCR and immunohistochemical or Western blot analyses

Global Trade Item Number

SKUGTIN
AP182R04053252373107

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