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Merck

T3260

Toluidine Blue O

Powder

Synonym(s):

Basic Blue 17, Blutene chloride, Methylene Blue T50 or T extra, Tolonium chloride

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About This Item

Empirical Formula (Hill Notation):
C15H16ClN3S
CAS Number:
92-31-9
Molecular Weight:
305.83
NACRES:
NA.47
PubChem Substance ID:
24900122
UNSPSC Code:
12171500
EC Number:
202-146-2
MDL number:
MFCD00011934
Colour Index Number:
52040

Product Name

Toluidine Blue O, Technical grade

InChI key

GEDVVYWLPUPJJZ-UHFFFAOYSA-N

InChI

1S/C15H15N3S.ClH/c1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;/h4-8,16H,1-3H3;1H

SMILES string

[Cl-].Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C

grade

technical grade

form

powder

composition

Dye content, ~80%

technique(s)

microbe id | staining: suitable

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Application

Toluidine Blue O has been used to stain a variety of specimens like mesenchymal cells, taste buds, osteoblasts, matrix, etc.

General description

A metachromatic dye suitable for a wide variety of histological staining procedures.
Toluidine Blue O (TBO) is a thiazine dye of the quinone-imine family and is cationic in nature. It has been employed in polychromatic staining of paraffin embedded plant cell walls. It is a metachromatic dye suitable for a wide variety of histological staining procedures.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SHBS5849
SHBR9627
0000289415
0000231220
0000289415

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Is there a difference between Toluidine Blue O and Toluidine Blue and which is best for staining tissue sections?

    Toluidine Blue O and Toluidine Blue are generally regarded as synonyms. The dye can be found in 2 forms. There is a chloride form and zinc chloride double salt.  Products T3260 and 198161 are the chloride form. This is the form certified by the Biological Stain Commission. The dye listed as simply Toluidine Blue, Product 89640, is the zinc chloride double salt. For routine use today, the chloride form should be utilized. The product number recommended for biological/histological staining is Product 198161, Toluidine Blue O.  This product has been found to provide acceptable staining of tissue by the Biological Stain Commission. Products 198161 and 89640 have not been suitability tested for the staining of tissue sections.

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